4.5 Article

Antimicrobial nano-assemblies of tryptocidine C, a tryptophan-rich cyclic decapeptide, from ethanolic solutions

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BIOCHIMIE
卷 204, 期 -, 页码 22-32

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ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2022.08.017

关键词

Antimicrobial cyclic peptide; Tryptocidine; Tyrothricin; Self-assembly; Nanocrystals; Morphology; Antimicrobial activity; Surface activity

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TpcC, a Trp-rich cyclodecapeptide, can oligomerize and form self-assembled nanoparticles in aqueous solutions. The solvent system of ethanol:water has an influence on TpcC self-assembly, with different structures observed based on ethanol concentration. The drying process from 20 to 50% ethanol resulted in polymorphic surface morphologies with various small nanoparticles, while drying from 75% ethanol led to highly facetted 3-D crystals. The TpcC nano-assemblies have potential applications in antimicrobial coatings, surface disinfectants, food packaging, and wound healing materials.
Tryptocidine C (TpcC), a Trp-rich cyclodecapeptide is a minor constituent in the antibiotic tyrothricin complex from Brevibacillus parabrevis. TpcC possesses a high tendency to oligomerise in aqueous solu-tions and dried TpcC forms distinct self-assembled nanoparticles. High-resolution scanning electron microscopy revealed the influence of different ethanol:water solvent systems on TpcC self-assembly, with the TpcC, dried from a high concentration in 15% ethanol, primarily assembling into small nano -spheres with 24.3 nm diameter and 0.05 polydispersity. TpcC at 16 mM, near its CMC, formed a variety of structures such as small nanospheres, large dense nanospheroids and facetted 3-D-crystals, as well as sheets and coarse carpet-like structures which depended on ethanol concentration. Drying 16 mM TpcC from 75% ethanol resulted in highly facetted 3-D crystals, as well as small nanospheres, while those in 10% ethanol preparation had less defined facets. Drying from 20 to 50% ethanol led to polymorphic ar-chitectures with a few defined nanospheroids and various small nanoparticles, imbedded in carpet-and sheet-like structures. These polymorphic surface morphologies correlated with maintenance of fluo-rescence properties and the surface-derived antibacterial activity against Staphylococcus aureus over time, while there was a significant change in fluorescence and loss in activity in the 10% and 75% preparations where 3-D crystals were observed. This indicated that TpcC oligomerisation in solutions with 20-50% ethanol leads to metastable structures with a high propensity for release of antimicrobial moieties, while those leading to crystallisation limit active moieties release. TpcC nano-assemblies can find application in antimicrobial coatings, surface disinfectants, food packaging and wound healing materials.(c) 2022 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

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