期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
卷 1868, 期 11, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbadis.2022.166509
关键词
Trytophan/kynurenine pathway; KMO; Type 2 diabetes; GK rat; Beta cells; Non-beta cells
资金
- French ANRT [2011/1223]
- Natural Science Foundation of Shandong province, China [ZR2020MH101]
- Metabrain Research
- Universite Paris-Cite
- National Health and Medical Research Council (NHMRC)
- Australian Research Council (ARC)
- Macquarie university
Type 2 diabetes is associated with inflammation in pancreatic islets. Inhibiting the tryptophan/kynurenine pathway can improve insulin secretion and glucose metabolism, suggesting a potential therapeutic strategy for type 2 diabetes.
Type 2 diabetes is associated with an inflammatory phenotype in the pancreatic islets. We previously demonstrated that proinflammatory cytokines potently activate the tryptophan/kynurenine pathway (TKP) in INS-1 cells and in normal rat islets. Here we examined: (1) the TKP enzymes expression in the diabetic GK islets; (2) the TKP enzymes expression profiles in the GK islets before and after the onset of diabetes; (3) The glucose-stimulated insulin secretion (GSIS) in vitro in GK islets after KMO knockdown using specific morpholino-oligonucleotides against KMO or KMO blockade using the specific inhibitor Ro618048; (4) The glucose tolerance and GSIS after acute in vivo exposure to Ro618048 in GK rats. We report a remarkable induction of the kmo gene in GK islets and in human islets exposed to proinflammatory conditions. It occurred prominently in beta cells. The increased expression and activity of KMO reflected an acquired adaptation. Both KMO knockdown and specific inhibitor Ro618048 enhanced GSIS in vitro in GK islets. Moreover, acute administration of Ro618048 in vivo improved glucose tolerance, GSIS and basal blood glucose levels in GK rats. These results demonstrate that targeting islet TKP is able to correct defective GSIS. KMO inhibition could represent a potential therapeutic strategy for type 2 diabetes.
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