Low-flux electron diffraction study on body site dependence of stratum corneum structures in human skin

For analysis of the structure of human skin stratum corneum (SC), we introduced low-flux electron diffraction (ED) and developed a new statistical analysis method for obtained ED intensity profiles. By use of this method we compared the differences in the intercellular lipid organization on the SC corneocytes collected at human forehead, cheek, and forearm by the grid-stripping method. As a result, we found a significant regional difference in the distribution of lipid hydrocarbon chain packing domains in the SC; the ring-type ED pattern with orthorhombic symmetry was more often observed in the forearm SC than in the forehead and cheek SCs. We also found that the dependence of the background electron diffraction intensity on the modulus of the scattering vector differed significantly among them. The present method for the analysis of a large number of ED patterns of noninvasively obtained SC samples could be a powerful tool to scrutinize the structural difference between the SCs under various experimental conditions.

Automated summary

In this study, low-flux electron diffraction and a statistical analysis method were introduced to compare the differences in intercellular lipid organization in the human skin stratum corneum from different areas. The results showed significant regional differences in the distribution of lipid hydrocarbon chain packing domains. The present method can be a powerful tool for scrutinizing the structural differences between skin stratum corneum under various conditions.