期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 624, 期 -, 页码 89-94出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2022.07.009
关键词
Apo-SorCS3; Cryo-EM structure; Conformation change; Dimerization
资金
- Medical Scientific Reuter Foundation of Guangdong Province of China [A2021333]
- Shenzhen Science and Technology Innovation Commission Project [JCYJ20190808120613189]
- Shenzhen Pea-cock Program-Project Development Fund [20190904141C]
This study determined the structural details of the human VPS10 domain-containing receptor SorCS3 and identified at least three distinct conformations in its apo state. Conserved amino acid residues may play key roles in its dimerization and protein/polypeptide binding, differentiating it from homologous proteins.
The human VPS10 domain-containing receptor SorCS3 belongs to the Vps10p-domain receptor family and is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the cryo-EM structure of the full-length SorCS3 receptor and further found that there were at least three distinct conformations (monomer, M-shaped dimer and N-shaped dimer) of SorCS3 in the apo state. The differences between the two dimer conformations were caused by PKD1-2 assembly. In contrast to its homologous proteins, the conserved residues GLN198, ARG678, TYR430, GLU1020 and ASP1024 may be key points for its dimerization and for protein/polypeptide binding. These results showed the structural details of apo-SorCS3, which provides a foundation for elucidating the mechanism of protein sorting. (c) 2022 Elsevier Inc. All rights reserved.
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