Pseudomonas marianensis sp. nov., a marine bacterium isolated from deep-sea sediments of the Mariana Trench

A novel marine Gram-stain-negative, aerobic, rod-shaped bacterium, designated as strain PS1(T), was isolated from the deep-sea sediments of the Mariana Trench and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 35 degrees C (ranging 10-45 degrees C), pH 6 (ranging pH 5-10) and with 11% (w/v) NaCl (ranging 0-17%). The 16S rRNA gene sequence similarity results revealed that strain PS1(T) was most closely related to Pseudomonas stutzeri ATCC 17588(T), Pseudomonas nitrititolerans GL14(T), Pseudomonas zhaodongensis NEAU-ST5-21(T), Pseudomonas xanthomarina DSM 18231(T) and Pseudomonas kunmingensis HL22-2(T) with 98.3-98.7%. The digital DNA-DNA hybridization values and the average nucleotide identity between strain PS1(T) and the reference strains were 20.4-40.1% and 78.7-79.4%, respectively. The major respiratory quinone is ubiquinone Q-9. The major polar lipids were phosphatidylethanolamine, diphosphatidyglycerol, phosphatidylglycerol, phosphatidylcholine, aminoglycolipid, two unidentified glycolipids and one unidentified lipid. The predominant cellular fatty acids of strain PS1(T) were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and cyclo-C-19:0 omega 8c. The G + C content of the genomic DNA was 63.0%. The combined genotypic and phenotypic data indicated that strain PS1(T) represents a novel species of the genus Pseudomonas, for which the name Pseudomonas marianensis sp. nov. is proposed, with the type strain PS1(T) (= DSM 112238(T) = MCCC 1K05112(T)).

Automated summary

A novel species of the genus Pseudomonas, named Pseudomonas marianensis sp. nov., was isolated from deep-sea sediments of the Mariana Trench. It showed distinct phylogenetic and phenotypic characteristics.