期刊
AQUATIC TOXICOLOGY
卷 250, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2022.106235
关键词
Ussing chamber; Polyethylene; MP; Translocation; Ingestion; Methodology
The present study demonstrates the ability of marine microplastics to cross the intestinal wall of sea urchins and enter the coelomic fluid. The size-dependence of microplastic translocation across the intestinal wall is also highlighted.
The fate and toxicity of ingested marine microplastics (MPs) have been of major concern in aquatic ecotoxicology for the last decade. Although their ingestion by a wide range of marine organisms has been proven, the uptake of MPs within organs is not yet fully understood and relies on the ability of ingested microplastics to transfer from the gut to tissues beyond the digestive wall (i.e., translocation). The present study investigates the in vitro transfer of fluorescent high-density polyethylene particles of different sizes classes (1-5 mu m; 10-29 mu m; 38-45 mu m) across the intestinal wall of the sea urchin Paracentrotus lividus using Ussing chambers. Small microplastics (1-5 mu m) were proven to be able to cross the intestinal wall of P. lividus and reach the coelomic fluid, while larger microplastics (>= 10 mu m) were not observed to cross the intestinal wall. Results demonstrate a size-dependent passage of polyethylene microparticles across the intestinal walls of P. lividus for the first time, highlighting the suitability of Ussing chamber systems to study the transfer of MPs across the intestinal wall of animals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据