期刊
APPLIED SURFACE SCIENCE
卷 596, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.apsusc.2022.153518
关键词
Biomaterials; Plasma polymerization; Surface chemistry; Albumin; Immune response; Conformational change
类别
资金
- NHMRC [GNT1194466]
- ARC [DP180101254]
The surface chemistry of biomaterials plays a crucial role in regulating protein adsorption, conformational changes, and immune responses. Positively charged amine and oxazoline-rich surface chemistries have the greatest impact on albumin adsorption and protein conformational changes. Pre-adsorption of albumin leads to reduced expression of pro-inflammatory cytokines and increased secretion of anti-inflammatory markers.
The surface chemistry of biomaterials plays a pivotal role in regulating the type, amount, and conformational changes of adsorbed proteins, which then modulates the subsequent innate immune responses. Albumin is the most abundant protein in serum and a major constituent of the protein corona that forms on biomaterial surfaces. Therefore, it is important to understand the role of surface chemistry in albumin adsorption, surface induced conformational changes, and how this affects the subsequent immune responses. To interrogate these events, we generated model substrata with four tailored surface chemistries rich in amines, oxazolines, carboxylic acid groups and pure hydrocarbons via plasma polymerization. The positively charged amine and oxazoline rich surface chemistries caused greatest albumin adsorption and protein conformational changes. Our data demonstrated that macrophages (differentiated THP-1 cells) were able to interact with unfolded albumin via their scavenger receptors. The innate inflammatory responses were studied by measuring the expression of pro- and anti- inflammatory markers. As an overall trend, the pre-adsorption of albumin resulted in a reduction in the level of expression of pro-inflammatory cytokines while the secretion of anti-inflammatory markers was stimulated. This study provides valuable information, which could aid in the design of future biomaterials that elicit predictable immune response.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据