期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 106, 期 22, 页码 7449-7460出版社
SPRINGER
DOI: 10.1007/s00253-022-12213-5
关键词
Bacterial cellulose; Biopolymers; Fruit; Apple juice; Novacetimonas; Acetic acid bacteria
资金
- Ministry of Agriculture and the Rural Support Service of the Republic of Latvia [19-00-A01612-000004]
Biomaterials and biopolymers, such as bacterial cellulose (BC), have become important sustainable materials with various potential applications. However, BC production faces challenges such as low yields and high costs. This study aims to evaluate BC synthesis using apple juice as a growth medium and find the optimal apple juice and beef extract concentrations to improve BC production.
Biomaterials and biopolymers, such as bacterial cellulose (BC), are becoming increasingly important as sustainable materials with a wide range of potential applications. However, BC industrial production is associated with several difficulties such as low BC production yields and high production costs; therefore, cheap alternative growth media, e.g. apple juice are being studied intensively. The aim of this study is to evaluate BC synthesis under static conditions on apple juice medium (AJM). The optimal concentration of apple juice in unsupplemented AJM for Novacetimonas hansenii MSCL 1646 was shown by its dilution 1:6 with water, which resulted in 0.89 +/- 0.01 g/L of dry BC weight after 10 cultivation days. Low BC synthesis can be associated with insufficient N concentration in apple juice; therefore, different organic and inorganic N sources were evaluated in combination with AJM, and beef extract (5 g/L) was found to be the most suitable. Further, AJM optimisation experiment showed the optimal apple juice and beef extract concentrations as 1:2 and 15 g/L respectively, which resulted in 17.27 +/- 0.07 g/L of dry BC weight, which is significantly higher than in standard Hestrin-Schramm (HS) medium (4.07 +/- 0.02 g/L). Analysis of mechanical and physical properties showed that use of AJM results in changes in BC properties compared with the standard HS medium. Results of the study indicate that apple juice is an effective and cheap C source that in combination with appropriate N source leads to high BC synthesis and makes it suitable for industrial BC production.
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