期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 106, 期 19-20, 页码 6745-6757出版社
SPRINGER
DOI: 10.1007/s00253-022-12151-2
关键词
Foot-and-mouth disease; Monoclonal antibody; Blocking ELISA; Non-structural protein antibodies
资金
- Consortia of Research Platforms (CRP) on Vaccines and Diagnostics Project (ICAR, New Delhi)
- Ministry of Agriculture, Nature and Food Quality, the Netherlands [WOT01-003-11]
This study developed a new NSP antibody blocking ELISA test and validated it on large panels of sera from different susceptible species. The test showed high diagnostic sensitivity and specificity, making it applicable for a wide range of animals.
Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations.
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