Antiproliferative Activity of Green Synthesised Silver Nanoparticles of Cassia Marginata Roxb

Green synthesised silver nanoparticles (AgNPs) have been identified as a promising new therapy pathway for cancer because of their anti-angiogenic potency. In the present study, CM-AgNPs were biosynthesized using aqueous leaf extract of Cassia marginata Roxb (CM) and were confirmed by spectral studies like UV-Visible and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic techniques. The structure and shape of the biosynthesized CM-AgNPs were confirmed by scanning electron microscope (SEM), energy-dispersive spectroscopy (EDX), and high-resolution transmission electron microscopy (HR-TEM) with selected area electron diffraction (SAED) studies. The antioxidant activity of the synthesized nanoparticles were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay methods, and the IC50 values were found to be 19.58 +/- 1.62 and 742.33 +/- 14.57 mu g, respectively. The anticancer activity of CM-AgNPs was evaluated against human breast cancer cell lines MCF-7 and MDA-MB-231 by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, and the IC50 values were found to be 21.02 +/- 1.67 and 26.21 +/- 0.33 mu g/ml, respectively. These results provide promising information about the biosynthesized CM-AgNPs that can be used in the development of novel anticancer drugs.

Automated summary

Green synthesised silver nanoparticles (AgNPs) have been successfully biosynthesised using Cassia marginata Roxb leaf extract. The CM-AgNPs were confirmed by spectral studies and exhibited antioxidant and anticancer activities, showcasing their potential for the development of novel anticancer drugs.