期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 316, 期 -, 页码 178-185出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2016.05.014
关键词
Sulfur dioxide; Arabidopsis; MicroRNA; Target gene; Deep sequencing
资金
- National Natural Science Foundation of China [30470318, 30870454, 31371868]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20121401110007]
- Shanxi Scholarship Council of China [2009022]
- China Postdoctoral Science Founded Project [2014M551056]
Sulfur dioxide (SO2) is a major air pollutant and has significant impacts on plants. MicroRNAs (miRNAs) are a class of gene expression regulators that play important roles in response to environmental stresses. In this study, deep sequencing was used for genome-wide identification of miRNAs and their expression profiles in response to SO2 stress in Arabidopsis thaliana shoots. A total of 27 conserved miRNAs and 5 novel miRNAs were found to be differentially expressed under SO2 stress. qRT-PCR analysis showed mostly negative correlation between miRNA accumulation and target gene mRNA abundance, suggesting regulatory roles of these miRNAs during SO2 exposure. The target genes of SO2-responsive miRNAs encode transcription factors and proteins that regulate auxin signaling and stress response, and the miRNAs-mediated suppression of these genes could improve plant resistance to SO2 stress. Promoter sequence analysis of genes encoding SO2-responsive miRNAs showed that stress-responsive and phytohormone-related cis-regulatory elements occurred frequently, providing additional evidence of the involvement of miRNAs in adaption to SO2 stress. This study represents a comprehensive expression profiling of SO2-responsive miRNAs in Arabidopsis and broads our perspective on the ubiquitous regulatory roles of miRNAs under stress conditions. (C) 2016 Elsevier B.V. All rights reserved.
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