期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 316, 期 -, 页码 221-231出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2016.04.070
关键词
Chlorothalonil; Glutathione; Mitogen-activated protein kinase; Nitric oxide; Solanum lycopersicum
资金
- Natural Science Foundation of China [31000905]
- Foundation for the Author of National Excellent Doctoral Dissertation of China [201263]
- Fundamental Research Funds of the Central University of China [2016FZA6013]
Nitric oxide (NO) and mitogen-activated protein kinase (MPK) play important roles in brassinosteroid (BR)-induced stress tolerance, however, their functions in BR-induced pesticides metabolism remain unclear. Here, we showed that MPK activity and transcripts of SIMPK1 and SIMPK2 were induced by chlorothalonil (CHT), a widely used fungicide, in tomato leaves. However, cosilencing of SIMPK1/2 compromised the 24-epibrassinolide (EBR)-induced upregulation of detoxification genes and CHT metabolism in tomato leaves. In addition, cosilencing of SIMPK1/2 inhibited the accumulation of S-nitrosothiol (SNO), the reservoir of nitric oxide (NO) in plants, whereas tungstate, the inhibitor of nitrate reductase (NR), blocked EBR-induced SNO accumulation and MPK activity. Inhibiting the accumulation of NO by cPTIO, the specific scavenger and tungstate abolished the EBR-induced upregulation of detoxification genes, glutathione accumulation and CHT metabolism. The results showed that MPK and NR-dependent NO were involved in BR-induced CHT metabolism. Notably, there was a positive crosstalk between the MPK and NO production. (C) 2016 Elsevier B.V. All rights reserved.
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