期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 307, 期 -, 页码 173-183出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2015.11.060
关键词
Sodium dodecyl sulfate; Oxidative stress; Catalase; Isothermal titration calorimetry; Multi-spectroscopic studies
资金
- NSFC, Cultivation Fund of the Key Scientific and Technical Innovation Project [20875055, 21277081, 21477067]
- Research Fund for the Doctoral Program of Higher Education
- Ministry of Education of China [708058, 20130131110016]
- Science and Technology Development Plan of Shandong Province [2014GSF117027]
- Fundamental Research Funds of Shandong University [2014BT013, 2015JC030]
- China Postdoctoral Science Foundation [2014M560555, 2015T80720]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry [2015311]
Sodium dodecyl sulfate (SDS) contributes to adverse effects of organisms probably because of its ability to induce oxidative stress via changing the activity of antioxidant enzyme catalase (CAT). But the underlying molecular mechanisms still remain unclear. This study characterized the harmful effects of SDS-induced oxidative stress on the mouse primary hepatocytes as well as the structure and function of CAT molecule and investigated the underlying molecular mechanism. After 12 h SDS (0.1 mu M to 0.2 mM) exposure, no significant change was observed in CAT activity of the hepatocytes. After 0.5 and 0.8 mM SDS exposure, the state of oxidative stress stimulated CAT production in the hepatocytes. The inhibition of CAT activity induced by directly interacting with SDS was unable to catch the synthesis of CAT and therefore resulted in the increased activity and elevated ROS level. Further molecular experiments showed that SDS prefers to bind to the interface with no direct effect on the active site and the structure of heme groups of CAT molecule. When the sites in the interface is saturated, SDS interacts with VAL 73, HIS 74, ASN 147 and PHE 152, the key residues of the enzyme activity, and leads to the decrease of CAT activity. (C) 2015 Elsevier B.V. All rights reserved.
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