期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 47, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202201848
关键词
F-19 NMR; DNA Structure; Fluorinated Nucleotides; Nucleic Acids
资金
- National Natural Science Foundation of China [91953115, 21877064]
- Natural Science Foundation of Tianjin City [20JCZDJC00830]
- Frontiers Science Center for New Organic Matter, Nankai University [63181206]
- Slovenian Research Agency (ARRS) [P1-0242, J1-1704]
- CERIC-ERIC consortium
- China Scholarship Council
Fluorinated nucleotides are crucial for F-19 NMR studies of nucleic acid structure and function. The synthesized 4'-SCF3-thymidine exhibits high adaptability and sensitivity, making it valuable for distinguishing diverse DNA structures with single-nucleotide resolution.
Fluorinated nucleotides are invaluable for F-19 NMR studies of nucleic acid structure and function. Here, we synthesized 4 '-SCF3-thymidine (T 4'-SCF3 ${{<^>{4{<^>\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4 '-SCF3 group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4 '-SCF3-DNA constitute an isolated spin system, offering high F-19 NMR sensitivity and excellent resolution of the positioning of T 4'-SCF3 ${{<^>{4{<^>\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T 4'-SCF3 ${{<^>{4{<^>\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ make it a valuable F-19 NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA.
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