期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 61, 期 46, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202211767
关键词
Photoactivatable Fluorophores; Photoinduced Electron Transfer; Single Molecule Imaging; Super-Resolution Imaging
资金
- National Natural Science Foundation of China [22074021, 22174025]
- Natural Science Foundation of Shanghai [21ZR1409100]
- Thousand Youth Talents Plan and Fudan University
Single molecule localization microscopy based on photoactivation has been developed as a powerful tool for investigating the ultrastructure of cells. A general strategy using the 2,3-dihydro-1,4-oxathiine group (SO) as a tag to attach to various skeletal structures was developed. The fluorophores generated singlet oxygen under excitation light, converting the SO moiety into its ester derivative and restoring fluorescence. This technique was successfully applied for time-lapse super-resolution tracking in living cells and super-resolution imaging of microtubule structures in neurons.
Single molecule localization microscopy based on photoactivation is a powerful tool for investigating the ultrastructure of cells. We developed a general strategy for photoactivatable fluorophores, using 2,3-dihydro-1,4-oxathiine group (SO) as a tag to attach to various skeletal structures, including coumarin, BODIPY, rhodamine, and cyanine. The conjugation of SO resulted in a significant loss of fluorescence due to photoinduced electron transfer (PeT). Under the irradiation of excitation light, singlet oxygen generated by the fluorophores converted the SO moiety into its ester derivative, terminated the PeT process, and restored the fluorescence. Single molecule localization imaging was achieved using a dual functional illuminating beam in the visible, acting as both the activating and the exciting source. We successfully applied these photoactivatable probes for time-lapse super-resolution tracking in living cells and super-resolution imaging of microtubule structures in neurons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据