期刊
ANALYTICAL CHEMISTRY
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.2c03658
关键词
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资金
- National Natural Science Foundation of China [22274089]
- National Key RAMP
- D Program of China [2019YFA0210100]
- Shandong Provincial Key Research and Development Program (Major Scientific and Technological Innovation Project) [2021CXGC010515]
- Jinan Innovation Team [2021GXRC034]
This study developed COF-DNA bicolor probes for simultaneous imaging of tumor-associated mRNAs and found that the nanoprobe prepared by the freezing method exhibited better performance with higher signal-to-noise ratio and signal intensity. It offers a promising tool for improved cancer diagnostic imaging.
Developing probes for the simultaneous detection of multiple tumor-associated mRNAs is beneficial for the precise diagnosis and early therapy of cancer. In this work, we prepared two COF-DNA bicolor probes at room temperature and freezing conditions and evaluated their performances in simultaneous imaging of intracellular tumor-associated mRNAs. By loading dye-labeled survivin- and TK1-mRNA recognition sequences on porphyrin COF NPs, nucleic acid-specific off-on nanoprobes were obtained. The nanoprobe prepared by the freezing method exhibits higher ssDNA loading density and better fluorescence quenching efficiency. Moreover, its signal-to-noise ratio is significantly higher than that prepared at room temperature, and the target recognition effect was unaffected. Significantly, the freezing-method-prepared nanoprobe has higher signal intensities in target-overexpressed cells compared to the room-temperature-prepared probe, while their signals in cells with low target expression are similar. Thus, the freezing-method-prepared nanoprobe is a promising tool for improved cancer diagnostic imaging. This work can offer new insights into the exploration of high-performance COF-based nanoprobes for multiple biomarker detection.
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