Multiclass determination of endocrine disruptors in urine by hollow fiber microporous membrane and liquid chromatography

A simple and rapid methodology was developed using hollow fiber membrane microporous and a 96-well plate system for a high throughput multiclass determination of endocrine disruptors in human urine (diclofenac, diazepam, carbamazepine, ibuprofen, naproxen, carbofuran, methyl parathion, 17-alpha-ethynyl estradiol, bisphenol A and benzophenone). The quantification and detection of the chemicals were carried out by an HPLC-diode array detector. The fixed conditions for carrying out the method optimization were 1.5 mL of sample and 300 mu L of solvent desorption. Multivariate and univariate models were applied to optimize the parameters of the method, achieving the following conditions: 20% diluted urine, 1-octanol of extraction solvent impregnated in the microporous membrane, 70 min extraction in pH 3.0 and 30 min with a mixture of 75% methanol and 25% acetonitrile (v/v) for the desorption. The R-2 were & LE; 0.9973 for ibuprofen. The LOD ranged from 3.3 to 16.7 ng mL(-1) and the LOQ from 10 to 50 ng mL(-1). Relative recoveries ranged from 71% to 126%. The repeatability (n = 3) ranged from 0.22% to 12.01%, and the intermediate precision (n = 9) ranged from 0.13% to 17.76%. The method presents a good alternative for the determination of different classes of compounds in human urine.

Automated summary

A simple and rapid methodology using hollow fiber membrane microporous and a 96-well plate system was developed for high throughput multiclass determination of endocrine disruptors in human urine. The quantification and detection of the chemicals were carried out by an HPLC-diode array detector, and multivariate and univariate models were applied for method optimization.