期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 68, 期 2, 页码 186-200出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2022-0236OC
关键词
lung vaccination; eosinophils; plasma cells; antibody production
Eosinophils play a crucial role in inducing a complete vaccine response, promoting antibody secretion in the early stage of the immune response. They migrate to the lungs and secrete cytokines to activate B cells, promoting antibody production. However, the absence of eosinophils does not impair late immune responses due to a compensating mechanism involving the Th17 pathway. This study reveals a novel role for eosinophils in lung mucosal vaccination, accelerating early immune responses while regulating IgA production in the late stages.
Eosinophils have been previously shown to be able to regulate early humoral responses during systemic vaccination. Here we investigated the role of eosinophils during pulmonary vaccination, comparing vaccine-induced responses in eosinophil-deficient (Delta dblGATA) and wild-type mice using a Th2 adjuvant. We observed that eosinophils were needed to induce a complete vaccine response, thereby eliciting specific antibody-secreting plasma cells in the regional lymph nodes and antibody secretion in the BAL at the early stage of the immune response. Reintroduction of eosinophils in the lungs of Delta dblGATA mice during the priming stage enhanced both specific IgM and IgG plasma cells but not specific IgA plasma cells. Upon vaccination, eosinophils migrated to the lungs and secreted cytokines involved in B-cell activation, which might promote antibody production. Importantly, however, the absence of eosinophils did not impair late immune responses in a prime/boost protocol because, in that setup, we uncovered a compensating mechanism involving a Th17 pathway. In conclusion, our data demonstrate for the first time a new role for eosinophils during lung mucosal vaccination, whereby they accelerate early immune responses (IgM and IgG) while regulating IgA production at the late stages.
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