期刊
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
卷 88, 期 6, 页码 -出版社
WILEY
DOI: 10.1111/aji.13638
关键词
amniotic fluid; brain inflammation; disparity; infection; OOC
资金
- NIH/NICHD [R01HD100729-01S1]
- National Institutes of Health/Office of the Director (OD)/National Institute of Allergy and Infectious Diseases (NIAID) [K12HD052023]
- Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
A study using an organ-on-chip model has found that race/ethnicity differences play a role in perinatal brain injury, but the fetal sex of neonates does not affect susceptibility to intraamniotic inflammation leading to neuroinflammation.
Problem Fetal neuroinflammation has been linked to preterm birth-related intraamniotic infection and inflammation; However, the contribution of fetal sex and maternal race/ethnicity is unknown. To determine if fetal sex and maternal race/ethnicity influence neuroinflammation, an organ-on-chip (OOC) model were established under normal or pathologic conditions utilizing amniotic fluid. Method of study OOC is composed of two-cell culture chambers connected by Type IV collagen-coated microchannels. Human fetal astroglia (SVGp12) and microglia (HMC3) were co-cultured at an 80:20 ratio in the inner chamber. The outer chamber contained amniotic fluid (AF) from male and female fetuses of White Hispanic (WH) and African-American (AA) pregnant women with or without lipopolysaccharide (LPS-100 ng/ml) and incubated for 48 h. Glial migration (brightfield microscopy), activation (Immunocytochemistry), and cytokine production (Luminex assays) were quantified and compared (N = 4 for each category of sex and race/ethnicity). Results In a pooled analysis, AF+LPS did not induce glial activation or inflammatory changes compared to AF alone. When stratified by sex, male AF+LPS promoted significant glial activation (high CD11b:p p < 0.01) compared to male AF without LPS; however, this was not associated with changes in pro-inflammatory cytokines. When stratified by race/ethnicity, AF+LPS induced glial activation in both groups, but a differential increase in pro-inflammatory cytokines was seen between WH and AA AF (WH-interleukin-1 beta: p p < 0.01). Conclusion This OOC model of fetal neuroinflammation has determined that race/ethnicity differences do exist for perinatal brain injury. The fetal sex of neonates was not a determining factor of susceptibility to intraamniotic inflammation leading to neuroinflammation.
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