期刊
ACS APPLIED MATERIALS & INTERFACES
卷 -, 期 -, 页码 -出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c13303
关键词
core-satellite nanoparticles; absorption modulation; lateral flow immunoassay; COVID-19; SARS-CoV-2; nucleocapsid protein; high sensitivity
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2021R1A2B5B03001417]
- Ministry of Trade, Industry and Energy (MOTIE)
- Korea Institute for Advancement of Technology (KIAT) [P0019785]
- [B0080904000203]
- Ministry of Health & Welfare (MOHW), Republic of Korea [P0019785] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A novel rapid detection method using gold nanoparticle assembled SiO2 core-satellite nanoparticles has been developed for sensitive detection of SARS-CoV-2. The method achieves highly sensitive detection within 20 minutes and significantly improves the detection sensitivity by utilizing wellgrown AuNP satellites.
The worldwide spread of coronavirus disease 2019 (COVID-19) highlights the need for rapid, simple, and accurate tests to detect various variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The antigen test, based on the lateral flow immunoassay (LFI), is a suitable first line of defense test that enables early identification and timely isolation of patients to minimize viral transmission among communities. However, it is generally less accurate than nucleic acid testing, and its sensitivity needs improvement. Here, a novel rapid detection method is designed to sensitively detect SARS-CoV-2 using isolated gold nanoparticle (AuNP)assembled SiO2 core-satellite nanoparticles (SiO2@Au CSNPs). Wellgrown AuNP satellites in the synthesis of SiO2@Au CSNPs significantly enhanced their light absorption, increased the detection sensitivity, and lowered the detection limit by 2 orders of magnitude relative to conventional gold colloids. The proposed system enabled highly sensitive detection of the SARS-CoV-2 nucleocapsid protein with a detection limit of 0.24 pg mL(-1) within 20 min. This is the first study to develop a highly sensitive antigen test using the absorption-modulated SiO2@Au CSNPs. Our findings demonstrate the capacity of this platform to serve as an effective sensing strategy for managing pandemic conditions and preventing the spread of viral infections.
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