期刊
ACS APPLIED MATERIALS & INTERFACES
卷 14, 期 35, 页码 40322-40330出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.2c10810
关键词
PNIPAAm; on-demand delivery; CQD; thermoresponsive; cellular uptake
资金
- Center for Emergent Functional Matter Science of National Yang Ming Chiao Tung University from The Featured Areas Research Center Program by the Ministry of Education (MOE) in Taiwan
- Ministry of Science and Technology of the Republic of China [MOST 109-2113-M-001-036-MY3, MOST 108-2221-E-009-133MY3]
This paper presents a thermoresponsive carbon quantum dot-loaded blend nanofiber sheet for on-demand delivery of organic, inorganic, and biological cargo in vitro cellular uptake. The nanofiber sheet can effectively improve the cellular uptake of cargo by increasing the local concentrations via applying thermal stimulation as the released mechanism.
Developing a smart responsive surface for on-demand delivery of organic, inorganic, and biological cargo in vitro cellular uptake is always in constant demand. Herein, we present carbon quantum dot (CQD)-loaded (poly(N-isopropylacrylamide) (PNIPAAm)/poly(methyl methacrylate (PMMA)) blend nanofiber sheets having a thermoresponsive nature. As a model cargo, fluorescent CQDs are used for the demonstration of the on-demand delivery mechanism. In addition, a thermoresponsive nature is produced by the PNIPAAm polymer in the nanofiber matrix while the PMMA polymer provides extra stability and firmness to the nanofibers against the sudden dissolution of the nanofibers in aqueous media. The synthesis of CQDs and their loading into a blend nanofiber matrix are confirmed using fluorescence spectrophotometry, transmission electron microscopy, and fluorescence microscopy. The morphologies and diameters of the nanofibers are analyzed by scanning electron microscopy. Burst effect analysis proves that 30% (w/w) PNIPAAm-containing nanofibers possess the highest stability with the least dissolution in aqueous media. Thermoresponsiveness of the nanofibers is further confirmed through water contact angle measurements. Quantitative fluorescence results show that more than 80% of loaded CQDs can be released upon thermal stimulation. The fluorescence micrographs reveal that the blend nanofiber sheets can effectively improve the cellular uptake of CQDs by simply increasing the local concentrations via applying thermal stimulation as the released mechanism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据