期刊
JOURNAL OF GENETICS AND GENOMICS
卷 43, 期 6, 页码 393-404出版社
SCIENCE PRESS
DOI: 10.1016/j.jgg.2016.03.008
关键词
CTP synthase; Metabolic enzyme; Cytoophidium; Glycolysis; Glutamine; Intracellular compartmentation; Saccharomyces cerevisiae
资金
- UK Medical Research Council
- China Scholarship Council-University of Oxford Scholarship
- Chinese Scholarship Council Studentship
- Malaysia Government Scholarship
- National Natural Science Foundation of China [11304372]
- Medical Research Council [MC_U137788471, MC_UU_12021/3] Funding Source: researchfish
- MRC [MC_UU_12021/3, MC_U137788471] Funding Source: UKRI
Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame GFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases (Ura7p and Ura8p) and two asparagine synthetases (Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus. Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.
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