期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 82, 期 2, 页码 110-115出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10327-016-0646-y
关键词
beta-tubulin gene; Cucurbit downy mildew; High resolution melting analysis; Pseudoperonospora cubensis; Pseudoperonospora humuli; Real-time PCR
资金
- Bio-industry Technology Development Program of iPET (Korea Institute of Planning and Evaluation for Technology In Food, Agriculture and Forestry) [111057-5]
To develop a molecular method to identify Pseudoperonospora cubensis in cucumber leaves with signs of downy mildew, we compared the nucleotide sequences of ribosomal DNA-internal transcribed spacer, cytochrome oxidase II, and beta-tubulin genes of P. cubensis and P. humuli isolates. Seven single nucleotide polymorphisms (SNPs) distinguished P. cubensis and P. humuli based on variations in beta-tubulin sequences, and one specific primer set was designed for further analysis. Real-time PCR and high resolution melting analysis showed that the primer set can be used to specifically identify P. cubensis in cucumber leaves with downy mildew.
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