期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 82, 期 3, 页码 121-131出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10327-016-0656-9
关键词
Alternaria alternata pathotypes; Global regulator; LaeA; Secondary metabolite; Host-specific toxin
资金
- Japanese Society for Promotion of Sciences [24.8246, 19108001, 23248007, 23380025, 26304025]
- Global COE Program Advanced Utilization of Fungus/Mushroom Resources for Sustainable Society in Harmony with Nature'' by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan
- Grants-in-Aid for Scientific Research [23380025, 26304025, 16H04874, 15K14662, 19108001, 23248007] Funding Source: KAKEN
The global regulator LaeA is required for the expression of biosynthetic genes for secondary metabolites in filamentous fungi such as Aspergillus nidulans. To learn more about the regulation of these genes in Alternaria alternata, which has at least seven pathogenic variants (pathotypes) that produce host-specific toxins (HSTs) and cause severe diseases in their host plants, we identified LaeA homologs encoding methyltransferase in tomato, strawberry and apple pathotypes of A. alternata, designating them AtLAE1, AsLAE1 and AaLAE1, respectively. In the AtLAE1-deleted mutant of the tomato pathotype (Delta AtLAE1), expression of the AAL-toxin biosynthetic gene ALT1 was lower than that in the wild type. Correspondingly, AAL-toxin production and virulence of the mutant were significantly lower as were spore production and hyphal growth. Similarly, the AsLAE1-deleted mutant of the strawberry pathotype (Delta AsLAE1) and the AaLAE1-deleted mutant of the apple pathotype (Delta AaLAE1) produced less of their host-specific toxin (AF- and AM-toxin, respectively), had reduced virulence on the host plant, and hyphal growth and sporulation were defective. Thus, the global regulator gene LaeA positively regulates HST biosynthesis, pathogenicity, growth and differentiation in A. alternata pathotypes.
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