期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 148, 期 2, 页码 147-159出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.201611586
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资金
- National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute grant [P01HL078931]
- NIH/National Institute of General Medical Sciences grant [R01GM110276]
- American Heart Association Scientist Development grant [14SDG20300018, 16POST27250284]
- Chilean government grants FONDECYT [ACT1104, 1120864]
- Millennium Scientific Initiative of the Chilean Ministry of Economy
Excitation-evoked calcium influx across cellular membranes is strictly controlled by voltage-gated calcium channels (Ca-V), which possess four distinct voltage-sensing domains (VSDs) that direct the opening of a central pore. The energetic interactions between the VSDs and the pore are critical for tuning the channel's voltage dependence. The accessory alpha(2)delta-1 subunit is known to facilitate Ca(V)1.2 voltage-dependent activation, but the underlying mechanism is unknown. In this study, using voltage clamp fluorometry, we track the activation of the four individual VSDs in a human L-type Ca(V)1.2 channel consisting of alpha(1C) and beta 3 subunits. We find that, without alpha(2)delta-1, the channel complex displays a right-shifted voltage dependence such that currents mainly develop at nonphysiological membrane potentials because of very weak VSD-pore interactions. The presence of alpha(2)delta-1 facilitates channel activation by increasing the voltage sensitivity (i.e., the effective charge) of VSDs I-III. Moreover, the alpha(2)delta-1 subunit also makes VSDs I-III more efficient at opening the channel by increasing the coupling energy between VSDs II and III and the pore, thus allowing Ca influx within the range of physiological membrane potentials.
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