期刊
JOURNAL OF GENE MEDICINE
卷 18, 期 1-3, 页码 3-15出版社
WILEY
DOI: 10.1002/jgm.2869
关键词
helper lipid; liposome; non viral vector; primary cell; transfection
资金
- 'Association Francaise contre les Myopathies' (Evry, France)
- BPIFrance financement (Paris, France)
- French government [ANR-10-IBHU-005]
- Nantes Metropole and the Pays de la Loire Region
- IHU-Cesti (Investissement d'Avenir) [ANR-10-IBHU-005]
- National Research Agency via the 'Investment Into The Future' program [ANR-10-IBHU-005]
Background To optimize synthetic gene delivery systems, there is a need to develop more efficient lipid formulations. Most cationic lipid formulations contain 'helper' neutral lipids because of their ability to increase DNA delivery, in particular by improving endosomal escape of DNA molecules via the pH-buffering effect of protonatable groups and/or fusion with the lipid bilayer of endosomes. Methods We evaluated the influence of the linker structure between the two oleyl chains in the helper lipid on transfection efficiency in cell lines, as well as in primary cells (hepatocytes/cardiomyocytes). We reported the synthesis of two new pH-buffering imidazole helper lipids characterized by a polar headgroup containing one (compound 6) or two (compound 5) imidazole groups and two oleyl chains linked by an amide group. We studied their association with the aminoglycoside lipidic derivative dioleylsuccinylparomomycin (DOSP), which contains two oleyl chains linked to the aminoglycoside polar headgroup via an amide function. We compared the morphology and transfection properties of such binary liposomes of DOSP/5 and DOSP/6 with those of liposomes combining DOSP with another imidazole-based dioleyl helper lipid (MM27) in which a phosphoramido group acts as a linker between the two oleyl chains and imidazole function. Results The phosphoramido linker in the helper lipid induces a major difference in terms of morphology and resistance to decomplexation at physical pH for DOSP/helper lipid complexes. Conclusions This hybrid dioleyl linker composition of DOSP/MM27 led to higher transfection efficiency in cell lines and in primary cells compared to complexes with homogeneous dioleyl linker. Copyright (C) 2015 John Wiley & Sons, Ltd.
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