期刊
HELIYON
卷 8, 期 6, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2022.e09759
关键词
Chitin; Black soldier fly; Hermetia illucens; Fish feed; Acid hydrolysis; Liquid chromatography tandem mass; spectrometry
资金
- Norwegian Research Council [15456 - 06]
- Aquafly [238997]
- integrated international study programme Erasmus Mundus EMQAL
- Institute of Marine Research (IMR), Bergen, Norway
Insects are a natural source of feed for fish, and the quantification of chitin is important for understanding its effect on fish health. This study evaluated a rapid quantitative method for chitin and successfully applied it to fish feed samples supplemented with chitin from black soldier fly larvae.
Insects are a natural source of feed for fish and have received more attention as a potential source of sustainable high-quality protein. However, contrasting results in different feeding trials have been ascribed to the chitin contained in the exoskeleton of insects and highlighted the importance of developing reliable methods for the quantification of chitin to draw meaningful conclusions about its effect on fish health. A rapid method based on the hydrolysis of chitin into glucosamine and further quantification by liquid chromatography tandem mass spectrometry is evaluated. The method offers good selectivity, linearity, limit of detection (1.08 x 10-5 % w/v or 5.38 x 10-4% w/w), limit of quantification (3.26 x 10-5 % w/v or 1.63 x 10-3 % w/w), trueness (88.39-109.29 %) and precision (2.24-10.72 %). The quantitative method was successfully applied to real samples of fish feed supplemented with chitin from black soldier fly (Hermetia illucens) larvae.
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