期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 21, 页码 6037-6049出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw361
关键词
Anther; Argonaute; microRNA; phasiRNA; rice; spikelet
资金
- US National Science Foundation Plant Genome Research Program [1339229]
- British Council's Global Innovation Initiative
- National Natural Science Foundation of China [31430009, 31110103915, 32322040, 31271698]
- China Innovative Research Team
- Ministry of Education
- Program of Introducing Talents of Discipline to Universities (111 Project) [B14016]
- National Key Basic Research Developments Program, Ministry of Science and Technology, China [2013CB126902]
- Science and Technology Commission of Shanghai Municipality [13JC1408200]
- Leading Scientist in Agriculture of Shanghai Municipality
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1649424] Funding Source: National Science Foundation
- The British Council [GII107] Funding Source: researchfish
Transcriptional analysis (mRNA and small RNA) of rice flower organs demonstrates correlations of 24-nt phased small RNAs with anther maturation and with three small RNA-binding Argonautes.Dissection of the genetic pathways and mechanisms by which anther development occurs in grasses is crucial for both a basic understanding of plant development and for examining traits of agronomic importance such as male sterility. In rice, MULTIPLE SPOROCYTES1 (MSP1), a leucine-rich-repeat receptor kinase, plays an important role in anther development by limiting the number of sporocytes. OsTDL1a (a TPD1-like gene in rice) encodes a small protein that acts as a cofactor of MSP1 in the same regulatory pathway. In this study, we analyzed small RNA and mRNA changes in different stages of spikelets from wild-type rice, and from msp1 and ostdl1a mutants. Analysis of the small RNA data identified miRNAs demonstrating differential abundances. miR2275 was depleted in the two rice mutants; this miRNA is specifically enriched in anthers and functions to trigger the production of 24-nt phased secondary siRNAs (phasiRNAs) from PHAS loci. We observed that the 24-nt phasiRNAs as well as their precursor PHAS mRNAs were also depleted in the two mutants. An analysis of co-expression identified three Argonaute-encoding genes (OsAGO1d, OsAGO2b, and OsAGO18) that accumulate transcripts coordinately with phasiRNAs, suggesting a functional relationship. By mRNA in situ analysis, we demonstrated a strong correlation between the spatiotemporal pattern of these OsAGO transcripts and phasiRNA accumulations.
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