期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 14, 页码 4169-4178出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw194
关键词
After-ripening; dormancy; PM19; pre-harvest sprouting; seed; synteny; Triticum; aestivum
资金
- UK Biotechnology and Biological Sciences Research Council (BBSRC)
- UK Agriculture and Horticulture Development Board (AHDB)
- KWS through LINK grant [BB/I01800X/1, BB/J004588/1, BB/J004596/1]
- Lantmannen through LINK grant [BB/I01800X/1, BB/J004588/1, BB/J004596/1]
- Limagrain through LINK grant [BB/I01800X/1, BB/J004588/1, BB/J004596/1]
- RAGT through LINK grant [BB/I01800X/1, BB/J004588/1, BB/J004596/1]
- BBSRC [BB/D007321/1]
- National Program of Sustainability I [LO1204]
- Czech Science Foundation [14-07164S]
- John Innes Foundation
- BBSRC [BB/I01800X/1, BBS/E/J/000C0628, BB/D007321/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0628, BB/I01800X/1, BB/D007321/1] Funding Source: researchfish
Phs-A1 confers resistance to sprouting in wheat by delaying the rate of seed dormancy loss and is distinct from the previously proposed PM19 candidate genes.The precocious germination of cereal grains before harvest, also known as pre-harvest sprouting, is an important source of yield and quality loss in cereal production. Pre-harvest sprouting is a complex grain defect and is becoming an increasing challenge due to changing climate patterns. Resistance to sprouting is multi-genic, although a significant proportion of the sprouting variation in modern wheat cultivars is controlled by a few major quantitative trait loci, including Phs-A1 in chromosome arm 4AL. Despite its importance, little is known about the physiological basis and the gene(s) underlying this important locus. In this study, we characterized Phs-A1 and show that it confers resistance to sprouting damage by affecting the rate of dormancy loss during dry seed after-ripening. We show Phs-A1 to be effective even when seeds develop at low temperature (13 A degrees C). Comparative analysis of syntenic Phs-A1 intervals in wheat and Brachypodium uncovered ten orthologous genes, including the Plasma Membrane 19 genes (PM19-A1 and PM19-A2) previously proposed as the main candidates for this locus. However, high-resolution fine-mapping in two bi-parental UK mapping populations delimited Phs-A1 to an interval 0.3 cM distal to the PM19 genes. This study suggests the possibility that more than one causal gene underlies this major pre-harvest sprouting locus. The information and resources reported in this study will help test this hypothesis across a wider set of germplasm and will be of importance for breeding more sprouting resilient wheat varieties.
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