期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 11, 页码 3237-3249出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw135
关键词
AGO1; development; EMS mutant; foxtail millet; growth; HYL1; map-based cloning; RNA-seq
资金
- National High Technology Research and Development Program of China (863 Program) [2013AA102603]
- National Natural Science Foundation of China [31301328, 31522040]
- Beijing Natural Science Foundation [6142019]
- Fundamental Research Funds of ICS-CAAS [2013007]
- China Agricultural Research System grant [CARS07-13.5-A02]
- Agricultural Science and Technology Innovation Program of CAAS
The C-terminus of SiAGO1b is an essential motif for the interaction between SiAGO1b and SiHYL1, and plays a key role in regulating growth, development and stress responses in Setaria italic.Foxtail millet (Setaria italica (L.) P. Beauv), which belongs to the Panicoideae tribe of the Poaceae, is an important grain crop widely grown in Northern China and India. It is currently developing into a novel model species for functional genomics of the Panicoideae as a result of its fully available reference genome sequence, small diploid genome (2n=18, similar to 510Mb), short life cycle, small stature and prolific seed production. Argonaute 1 (AGO1), belonging to the argonaute (AGO) protein family, recruits small RNAs and regulates plant growth and development. Here, we characterized an AGO1 mutant (siago1b) in foxtail millet, which was induced by ethyl methanesulfonate treatment. The mutant exhibited pleiotropic developmental defects, including dwarfing stem, narrow and rolled leaves, smaller panicles and lower rates of seed setting. Map-based cloning analysis demonstrated that these phenotypic variations were attributed to a C-A transversion, and a 7-bp deletion in the C-terminus of the SiAGO1b gene in siago1b. Yeast two-hybrid assays and BiFC experiments revealed that the mutated region was an essential functional motif for the interaction between SiAGO1b and SiHYL1. Furthermore, 1598 differentially expressed genes were detected via RNA-seq-based comparison of SiAGO1b and wild-type plants, which revealed that SiAGO1b mutation influenced multiple biological processes, including energy metabolism, cell growth, programmed death and abiotic stress responses in foxtail millet. This study may provide a better understanding of the mechanisms by which SiAGO1b regulates the growth and development of crops.
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