期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 67, 期 8, 页码 2353-2366出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw042
关键词
DCP1; decapping; eIF2alpha; NB-LRR; P-bodies; RNA silencing; translation
资金
- National Science and Engineering Council of Canada (NSERC)
- CONACYT
- BBSRC [BBS/E/T/000PR5885, BBS/E/T/000PR6193] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR6193, BBS/E/T/000PR5885] Funding Source: researchfish
This report demonstrates that NB-LRR signaling leads to the specific repression of viral transcripts, which in turn leads to the induction of processing bodies (PBs). At the same time, it demonstrates that multiple pathways can lead to PB formation through different mechanisms.Plant NB-LRR proteins confer resistance to multiple pathogens, including viruses. Although the recognition of viruses by NB-LRR proteins is highly specific, previous studies have suggested that NB-LRR activation results in a response that targets all viruses in the infected cell. Using an inducible system to activate NB-LRR defenses, we find that NB-LRR signaling does not result in the degradation of viral transcripts, but rather prevents them from associating with ribosomes and translating their genetic material. This indicates that defense against viruses involves the repression of viral RNA translation. This repression is specific to viral transcripts and does not involve a global shutdown of host cell translation. As a consequence of the repression of viral RNA translation, NB-LRR responses induce a dramatic increase in the biogenesis of RNA processing bodies (PBs). We demonstrate that other pathways that induce translational repression, such as UV irradiation and RNAi, also induce PBs. However, by investigating the phosphorylation status of eIF2 alpha and by using suppressors of RNAi we show that the mechanisms leading to PB induction by NB-LRR signaling are different from these stimuli, thus defining a distinct type of translational control and anti-viral mechanism in plants.
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