Production, structure, and bioactivity of polysaccharide isolated from Tremella fuciformis

Tremella fuciformis polysaccharide (TFP) is an important bioactive substance in T. fuciformis, which contributes to its application as medicine and food. In the present work, TFP was extracted with enzymatic combined with wet beating and its physicochemical as well as biological activities were investigated in the study. The result showed that the purity of TFP1 reached 92.14% (m/m). The TFP1, purified by DEAE column, had a molecular weight (Mw) of 5.8 x 103 kDa and consisted mainly of mannose, xylose, fucose, glucose and glucuronic acid at a molar ratio of 1.91 : 0.1 : 2.49 : 6.23 : 0.95. The structure of TFP1 was preliminarily investigated by infrared spectroscopy, nuclear magnetic resonance and atomic force microscope (AFM) analysis. Moreover, the antioxidant assays showed that TFP1 could scavenge DPPH, ABTS+ and hydroxyl radicals. The excellent water holding capacity of TFP1 implied its application in the food, pharmaceutical andcosmetic industries. (c) 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Automated summary

This study extracted Tremella fuciformis polysaccharide (TFP) and investigated its physicochemical and biological activities. The results showed that TFP had high purity, moderate molecular weight, and contained various amino acids and carbohydrates. It exhibited excellent antioxidant and water holding capacity, indicating its potential applications in the pharmaceutical, food, and cosmetic industries.