Extraction of Reduced Infrared Biomarker Signatures for the Stratification of Patients Affected by Parkinson's Disease: An Untargeted Metabolomic Approach

An untargeted Fourier transform infrared (FTIR) metabolomic approach was employed to study metabolic changes and disarrangements, recorded as infrared signatures, in Parkinson's disease (PD). Herein, the principal aim was to propose an efficient sequential classification strategy based on SELECT-LDA, which enabled optimal stratification of three main categories: PD patients from subjects with Alzheimer's disease (AD) and healthy controls (HC). Moreover, sub-categories, such as PD at the early stage (PDI) from PD in the advanced stage (PDD), and PDD vs. AD, were stratified. Every classification step with selected wavenumbers achieved 90.11% to 100% correct assignment rates in classification and internal validation. Therefore, selected metabolic signatures from new patients could be used as input features for screening and diagnostic purposes.

Automated summary

An untargeted FTIR metabolomic approach was used to study metabolic changes in Parkinson's disease. A classification strategy based on SELECT-LDA was proposed, achieving high correct assignment rates in distinguishing PD patients from AD patients and healthy controls, as well as stratifying different stages of PD and differentiating between PDD and AD. The selected metabolic signatures could be used for screening and diagnosis.