4.5 Review

Optical Immunoassays Methods in Protein Analysis: An Overview

期刊

CHEMOSENSORS
卷 10, 期 8, 页码 -

出版社

MDPI
DOI: 10.3390/chemosensors10080326

关键词

immunoassay; optical detection; sensor; antibody; antigen; analysis; fluorescence; electrochemiluminescence; SERS; ELISA; COVID-19

资金

  1. Deutsche Forschungsgemeinschaft (DFG) [RI 2635/6-1, 464509280]

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Immunoassays, particularly those based on optical readout, have gained significant research attention in the field of sensors due to their high sensitivity. This review provides an overview of the basic concepts and practical examples of optical immunoassay techniques, aiming to assist readers in selecting the most useful techniques for their analyses. Special emphasis is placed on the application of immunoassays for detecting the SARS-CoV-2 virus.
Immunoassays are analytical tools that attract growing research attention in the field of sensors. Among the different analytical methods, the immunoassays based on optical readout have an important role due to the high sensitivity reached in past years by the instrumentation as well as by the preparation of new labels. This review aims to give an overview in term of basic concepts and practical examples of the most used optical immunoassays techniques, in order to help readers to choose the most useful techniques for their analyses. Particular emphasis is dedicated to the application of the presented immunoassays on the detection of the SARS-CoV-2 virus.

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