期刊
BIOSENSORS-BASEL
卷 12, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/bios12070507
关键词
bacteria; Salmonella typhimurium; Escherichia coli; Mach-Zehnder Interferometry; immunosensor
资金
- program of Industrial Scholarships of Stavros Niarchos Foundation
- European Union [101007299]
- Marie Curie Actions (MSCA) [101007299] Funding Source: Marie Curie Actions (MSCA)
In this study, a miniaturized immunosensor based on broadband Mach-Zehnder Interferometry was developed for the simultaneous determination of S. typhimurium and E. coli O157:H7 in drinking water and milk. The assay was fast, accurate, sensitive, and reproducible, making it suitable for on-site bacteria determination in these samples.
The consumption of water and milk contaminated with bacteria can lead to foodborne disease outbreaks. For this reason, the development of rapid and sensitive analytical methods for bacteria detection is of primary importance for public health protection. Here, a miniaturized immunosensor based on broadband Mach-Zehnder Interferometry for the simultaneous determination of S. typhimurium and E. coli O157:H7 in drinking water and milk is presented. For the assay, mixtures of bacteria solutions with anti-bacteria-specific antibodies were run over the chip, followed by solutions of biotinylated anti-species-specific antibody and streptavidin. The assay was fast (10 min for water, 15 min for milk), accurate, sensitive (LOD: 40 cfu/mL for S. typhimurium; 110 cfu/mL for E. coli) and reproducible. The analytical characteristics achieved combined with the small chip size make the proposed biosensor suitable for on-site bacteria determination in drinking water and milk samples.
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