4.6 Article

Structural Characterization and Anti-inflammatory Activity of a Galactorhamnan Polysaccharide From Citrus medica L. var. sarcodactylis

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FRONTIERS IN NUTRITION
卷 9, 期 -, 页码 -

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FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.916976

关键词

Citrus medica L. var. sarcodactylis; galactorhamnan polysaccharide; anti-inflammatory activity; antioxidant activity; structure characterization

资金

  1. key project at the central government level: the ability establishment of sustainable use for valuable Chinese medicine resources [2060302]
  2. Traditional Chinese Medicine Bureau of Guangdong Province [20212123]
  3. Medical Scientific Research Foundation of Guangdong Province, China [B2021357]

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This study successfully extracted a new polysaccharide K-CMLP from Citrus medica L. var. sarcodactylis and analyzed its structure and bioactivities. The results showed that K-CMLP exhibited strong free-radical-scavenging properties and inhibited the production of pro-inflammatory cytokines and reactive oxygen species.
This study aimed to extract polysaccharides from Citrus medica L. var. sarcodactylis (finger citron fruits) and analyze their structures and potential bioactivities. A new polysaccharide named K-CMLP was isolated and purified by Diethylaminoethylcellulose (DEAE)-Sepharose Fast Flow and DEAE-52 cellulose column chromatography with an average molecular weight of 3.76 x 10(3) kDa. Monosaccharide composition analysis revealed that K-CLMP consisted of rhamnose, galactose, and glucose, with a molar ratio of 6.75:5.87:1.00. Co-resolved by methylation and two-dimensional nuclear magnetic resonance (NMR), K-CLMP was alternately connected with 1, 2-Rha and 1, 4-Gal to form the backbone, and a small number of glucose residues was connected to O-4 of rhamnose. The results of DPPH center dot and ABTS(+)center dot radical scavenging assays indicated that both crude polysaccharide Citrus medica L. var. polysaccharide (CMLP) and K-CLMP exhibited strong free-radical-scavenging properties in a dose-dependent manner. In addition, K-CMLP significantly inhibited the production of pro-inflammatory cytokines (IL-6 and TNF-alpha) and reactive oxygen species (ROS) in RAW 264.7 cells treated with LPS. These results provide a basis for further use as one of the potential functions of food or natural medicine.

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