期刊
FRONTIERS IN NUTRITION
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fnut.2022.947706
关键词
elderberry; polysaccharide; separation and purification; structural characterization; antioxidant
资金
- key research and development plan of Shandong Province [2020LZGC0905]
This study investigated the isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits. The results showed that the elderberry polysaccharides exhibited significant antioxidant activity and could potentially be used as antioxidants in functional foods.
The isolation, purification, and antioxidant activity of polysaccharides extracted from elderberry fruits were studied. Two neutral polysaccharides (EFP-0 and EFP-1) and three acidic polysaccharides (EFP-2, EFP-3, and EFP-4) were isolated from elderberry. EFP-0, EFP-1, EFP-2, EFP-3, and EFP-4 all contain arabinose, galactose, glucose, and mannose, with molecular weights of 1.7981 x 10(6), 7.0523 x 10(6), 7.7638 x 10(6), 4.3855 x 10(5), and 7.3173 x 10(5) Da, respectively. Structural characterization showed that the backbone of EFP-2 consisted of -> 4)-Manp (1 -> 4)-beta-D-Glcp (1 -> and -> 4)-beta-D-Glcp (1 -> 5)-alpha-L-Araf (1 -> units, and T-alpha-L-Araf (1 -> and T-beta-D-Galp (1 -> residues were detected by methylation analysis and NMR analysis. In addition, the MTT assay and zebrafish oxidative damage assay showed that EFP-2 had a protective effect on H2O2-damaged RAW264.7 cells in a dose-dependent manner, and zebrafish with the addition of EFP-2 would have low levels of ROS in vivo which showed significant antioxidant activity. Therefore, the results showed that the elderberry polysaccharides have antioxidant activity and can be used as potential antioxidants in functional foods.
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