Longitudinal changes of lactopontin (milk osteopontin) in term and preterm human milk

BackgroundLactopontin (LPN) in breast milk, also known as milk osteopontin is thought to play a myriad of important roles in infants when they are immature. The purpose of the present study was to examine the longitudinal changes in LPN concentrations in term and preterm milk, and elucidate the links between maternal characteristics, LPN levels, and child growth in a birth cohort. Methods131 mothers who delivered term, moderate-late preterm (MPT), very preterm (VPT), and extremely preterm (EPT) infants were included, milk samples were collected at 7, 14, 28, and 120 days postpartum. LPN concentration was determined by multiple reaction monitoring (MRM) using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). ResultsOur results indicated that LPN change over time of VPT (P = 0.024) and EPT (P = 0.003) were significantly different from term milk, although they all gradually decreased with lactation. In terms of LPN-related factors, maternal age was a significant contributor in late mature milk and pre-pregnancy BMI a significant contributor to colostrum and transitional milk. We further investigated relationships between LPN levels and infant weight and our results suggested that high levels of LPN in breast milk might be useful for the catch-up growth of infants. ConclusionLPN levels in breast milk are related to maternal factors, and differences in LPN levels may affect the growth of infants. As milk is a critical part in the mother-breastmilk-infant triad, the association between maternal-infant factors and milk LPN levels warrants further study.

Automated summary

This study examined the longitudinal changes in lactopontin (LPN) concentrations in the milk of preterm and term infants and explored the links between maternal characteristics, LPN levels, and child growth. The results showed that LPN levels gradually decreased with lactation, and LPN change over time differed significantly between preterm and term milk. Maternal age and pre-pregnancy BMI were found to be significant contributors to LPN levels. Higher levels of LPN in breast milk may be beneficial for infant catch-up growth.