期刊
BIOLOGY-BASEL
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/biology11081097
关键词
cyclodextrin; K2P channels; K+ leak currents; cerebellar granule neurons
类别
资金
- Fondecyt [1211838, 1191133]
- FIC-R
Two pore domain potassium channels play an important role in excitability in the nervous system. These channels produce background K+ currents and are associated with lipid rafts, but their functional activity is independent of cholesterol membrane organization.
Two pore domain potassium channels (K2P) are strongly expressed in the nervous system (CNS), where they play a central role in excitability. These channels give rise to background K+ currents, also known as IKSO (standing-outward potassium current). We detected the expression in primary cultured cerebellar granule neurons (CGNs) of TWIK-1 (K2P1), TASK-1 (K2P3), TASK-3 (K2P9), and TRESK (K2P18) channels by immunocytochemistry and their association with lipid rafts using the specific lipids raft markers flotillin-2 and caveolin-1. At the functional level, methyl-beta-cyclodextrin (M beta CD, 5 mM) reduced IKSO currents by similar to 40% in CGN cells. To dissect out this effect, we heterologously expressed the human TWIK-1, TASK-1, TASK-3, and TRESK channels in HEK-293 cells. M beta CD directly blocked TASK-1 and TASK-3 channels and the covalently concatenated heterodimer TASK-1/TASK-3 currents. Conversely, M beta CD did not affect TWIK-1- and TRESK-mediated K+ currents. On the other hand, the cholesterol-depleting agent filipin III did not affect TASK-1/TASK-3 channels. Together, the results suggest that neuronal background K+ channels are associated to lipid raft environments whilst the functional activity is independent of the cholesterol membrane organization.
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