期刊
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
卷 35, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13046-016-0371-6
关键词
FBXW7; Hepatocellular carcinoma; miR-155-3p
类别
资金
- National Natural Science Foundation of China [81360367, 81160066, 30870719]
- Scientific Research Foundation for Returned Scholars, Ministry of Education of China [jyb2010-01]
- Major Project of Science Research of Guangxi Universities [2013ZD046]
- Natural Science Foundation of Guangxi [2014GXNSFBA118162]
- Special Project of Traditional Chinese Medicine of Guangxi Health Department [GZPT13-45]
- Guangxi Distinguished Experts Special Fund
- Guangxi culture of new century academic and technical leader of special funds
Background: MicroRNAs (miRNAs) are small non-coding RNAs frequently dysregulated in human malignant tumors. In the present study, we analyzed the role miR-155-3p plays in Hepatocellular carcinoma (HCC), which has been reported participation in some other types of cancer. Methods: qRT-PCR was used to measure the levels of miR-155-3p in HCC specimens and HCC cell lines. Overexpression of miR-155-3p and miR-155-3p inhibitor were transfected into HCC cell lines to investigate its role in HCC. Colony formation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays were used to analyses cell proliferation in vitro. In vivo tumor formation assays were performed in BALB/c nude mice. Luciferase reporter assay was carried out to measure the translation of F-Box and WD repeat romain containing 7 (FBXW7). Results: We found that miR-155-3p was remarkably upregulated both in HCC tissue and cell lines. Overexpression of miR-155-3p enhanced HCC cell proliferation in vitro and tumorigenesis in vivo. In addition, overexpression of miR-155-3p is correlated with decreased levels FBXW7 mainly through inhibiting the expression of FBXW7. Conclusions: Our studies suggest that miR-155-3p plays an important role in the pathogenesis of HCC and implicates its potential applications in the treatment of HCC cancer.
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