期刊
COMMUNICATIONS BIOLOGY
卷 5, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s42003-022-03786-y
关键词
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资金
- Japan Society for the Promotion of Science (JSPS) [16J09035]
- JST SPRING [JPMJSP2128]
- JSPS KAKENHI [JP25291041, JP15H01359, JP16H04787, JP16H01317, JP18K19347, JP21H00261]
- Uehara Memorial Foundation
- Ohsumi Frontier Science Foundation
- Waseda University [2017B-242, 2017B-243, 2018B-222, 2019C-570, 2020R-038, 2022C-164]
- JSPS Core-to-Core Program
This study reveals that the inner kinetochore proteins Mis6 (CENP-I) and Mis15 (CENP-N) play a crucial role in maintaining the presence of CENP-A during mitosis in fission yeast. They achieve this by blocking the indiscriminate transcription of non-coding RNAs at the core centromere region.
Centromeres are established by nucleosomes containing the histone H3 variant CENP-A. CENP-A is recruited to centromeres by the Mis18-HJURP machinery. During mitosis, CENP-A recruitment ceases, implying the necessity of CENP-A maintenance at centromeres, although the exact underlying mechanism remains elusive. Herein, we show that the inner kinetochore protein Mis6 (CENP-I) and Mis15 (CENP-N) retain CENP-A during mitosis in fission yeast. Eliminating Mis6 or Mis15 during mitosis caused immediate loss of pre-existing CENP-A at centromeres. CENP-A loss occurred due to the transcriptional upregulation of non-coding RNAs at the central core region of centromeres, as confirmed by the observation RNA polymerase II inhibition preventing CENP-A loss from centromeres in the mis6 mutant. Thus, we concluded that the inner kinetochore complex containing Mis6-Mis15 blocks the indiscriminate transcription of non-coding RNAs at the core centromere, thereby retaining the epigenetic inheritance of CENP-A during mitosis. The kinetochore protein Mis6 (CENP-I) plays an important role in CENP-A maintenance during mitosis in fission yeast and blocks the indiscriminate transcription of non-coding RNAs at the core centromere to retain CENP-A during mitosis.
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