4.7 Article

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemistry & Molecular Biology

Orthogonal fluorescent chemogenetic reporters for multicolor imaging

Alison G. Tebo et al.

Summary: Spectrally separated fluorophores enable the observation of multiple targets in living cells simultaneously for a deeper understanding of cell functions. Engineered fluorogen-activating tags based on the FAST system offer orthogonality in recognition and spectral properties, allowing efficient multicolor imaging of proteins in live cells.

NATURE CHEMICAL BIOLOGY (2021)

Article Chemistry, Multidisciplinary

Color Tuning of Fluorogens for FAST Fluorogen-Activating Protein

Ivan N. Myasnyanko et al.

Summary: By using benzylidene imidazolone core, a range of color-shifted fluorogenic ligands for FAST protein were developed without compromising binding efficiency or utility for live-cell protein labeling. This study emphasizes the potential of benzylidene imidazolone derivatives for rapidly expanding live-cell fluorogenic labeling tools.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Article Multidisciplinary Sciences

Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging

Hela Benaissa et al.

Summary: Fluorescent reporters covering the visible spectrum are essential for imaging living cells and organisms. The authors have developed a collection of fluorogenic chromophores spanning from blue to red using a single engineered protein tag. This tool allows for tuning fluorescence color and properties, providing versatility for imaging proteins in live cells and optimizing FRET biosensors.

NATURE COMMUNICATIONS (2021)

Article Chemistry, Multidisciplinary

Developing Bright Green Fluorescent Protein (GFP)-like Fluorogens for Live-Cell Imaging with Nonpolar Protein-Chromophore Interactions

Cheng Chen et al.

Summary: A new fluorogen engineered from GFP chromophore by a unique strategy, exhibits over 550-fold fluorescence enhancement and high extinction coefficient upon binding to FAST. The large fluorescence enhancement is attributed to nonpolar protein-fluorogen interactions. This deep insight into structure-function relationships could guide the rational design of bright fluorogens for live-cell imaging with extended spectral properties.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

Article Chemistry, Multidisciplinary

NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids

Konstantin S. Mineev et al.

Summary: The paper discusses the impact of tag size on bioscience and medical applications, and presents a study on the structure of the fluorogen-activating protein FAST using NMR spectroscopy and X-ray crystallography. By designing a shortened version of the FAST protein, the researchers created the shortest genetically encoded tag among known fluorescent and fluorogen-activating proteins.

CHEMICAL SCIENCE (2021)

Article Chemistry, Multidisciplinary

A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo Molecular Imaging

Chenge Li et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Review Chemistry, Organic

Engineering Glowing Chemogenetic Hybrids for Spying on Cells

Hela Ben Aissa et al.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2020)

Review Chemistry, Multidisciplinary

Illuminating Cellular Biochemistry: Fluorogenic Chemogenetic Biosensors for Biological Imaging

Fanny Broch et al.

CHEMPLUSCHEM (2020)

Article Chemistry, Multidisciplinary

Red-Shifted Substrates for FAST Fluorogen-Activating Protein Based on the GFP-Like Chromophores

Natalia V. Povarova et al.

CHEMISTRY-A EUROPEAN JOURNAL (2019)

Article Multidisciplinary Sciences

A split fluorescent reporter with rapid and reversible complementation

Alison G. Tebo et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy

Alison G. Tebo et al.

BIOCHEMISTRY (2018)

Article Biochemical Research Methods

High-throughput, image-based screening of pooled genetic-variant libraries

George Emanuel et al.

NATURE METHODS (2017)

Review Biochemistry & Molecular Biology

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Erik A. Rodriguez et al.

TRENDS IN BIOCHEMICAL SCIENCES (2017)

Article Multidisciplinary Sciences

Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo

Marie-Aude Plamont et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Review Biochemistry & Molecular Biology

Novel uses of fluorescent proteins

Alexander S. Mishin et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2015)

Article Biochemical Research Methods

Relative and absolute determination of fluorescence quantum yields of transparent samples

Christian Wuerth et al.

NATURE PROTOCOLS (2013)

Review Physiology

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

Dmitriy M. Chudakov et al.

PHYSIOLOGICAL REVIEWS (2010)

Article Biochemistry & Molecular Biology

Improved side-chain torsion potentials for the Amber ff99SB protein force field

Kresten Lindorff-Larsen et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2010)

Article Biochemistry & Molecular Biology

Efficient analysis of macromolecular rotational diffusion from heteronuclear relaxation data

P Dosset et al.

JOURNAL OF BIOMOLECULAR NMR (2000)