SIPGCN: A Novel Deep Learning Model for Predicting Self-Interacting Proteins from Sequence Information Using Graph Convolutional Networks

Protein is the basic organic substance that constitutes the cell and is the material condition for the life activity and the guarantee of the biological function activity. Elucidating the interactions and functions of proteins is a central task in exploring the mysteries of life. As an important protein interaction, self-interacting protein (SIP) has a critical role. The fast growth of high-throughput experimental techniques among biomolecules has led to a massive influx of available SIP data. How to conduct scientific research using the massive amount of SIP data has become a new challenge that is being faced in related research fields such as biology and medicine. In this work, we design an SIP prediction method SIPGCN using a deep learning graph convolutional network (GCN) based on protein sequences. First, protein sequences are characterized using a position-specific scoring matrix, which is able to describe the biological evolutionary message, then their hidden features are extracted by the deep learning method GCN, and, finally, the random forest is utilized to predict whether there are interrelationships between proteins. In the cross-validation experiment, SIPGCN achieved 93.65% accuracy and 99.64% specificity in the human data set. SIPGCN achieved 90.69% and 99.08% of these two indicators in the yeast data set, respectively. Compared with other feature models and previous methods, SIPGCN showed excellent results. These outcomes suggest that SIPGCN may be a suitable instrument for predicting SIP and may be a reliable candidate for future wet experiments.

Automated summary

Protein is the fundamental organic substance in cells that plays a crucial role in biological activities. Self-interacting protein (SIP) is an important protein interaction. This study presents a SIP prediction method, SIPGCN, using a deep learning graph convolutional network (GCN). The results demonstrate excellent performance of SIPGCN.