期刊
NANOIMPACT
卷 27, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.impact.2022.100412
关键词
Human serum albumin; Polystyrene; Nanoplastic; Fluorescence spectroscopy; Thermodynamic analysis
资金
- Indian Council of Medical Research (ICMR) [36/2/2020/Toxi/BMS]
This study focuses on the interaction between polystyrene nanoplastics and human serum albumin, resulting in changes in the structure and function of the protein. The study highlights the need for regulation of nanoplastics in consumer products and further research on their fate in the biological system.
Nanoplastics finds its presence in most of the consumer products. Their chance of coming in contact with human cells and components is rampant. This study focuses on the interaction of polystyrene nanoplastics (PSNPs) with human serum albumin (HSA), ultimately causing structural and functional properties of the protein. Fluores-cence and UV-Visible spectroscopic studies reported that PSNPs form a spontaneous ground-state complex with HSA, by hydrogen bonding, van der waal's, and hydrophobic force of attraction. This causes changes in the environment around major aromatic amino acids, especially tryptophan-214, which has a strong affinity with PSNPs. Further docking analysis confirmed hydrophobic interactions between PSNPs and aromatic amino acids in subdomain IIA of HSA. A shift in amide bands in HSA, as determined by FTIR analysis confirmed the dis-turbances in its secondary structure followed by reordering which will lead to the unfolding of HSA. Besides, PSNPs reduce the esterase activity of HSA by competitive inhibition. This molecular-level information such as binding energy, binding site, binding forces, reversible or irreversible binding, and structural changes of protein will shed light on the extent of toxicity in humans. This study will emphasize the urgent need for regulation of the use of nanoplastics (NPs) in consumer products, as well as the need for more research to determine the fate of NPs in the biological system.
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