☆ 4.7 Review

An updated review of SARS-CoV-2 detection methods in the context of a novel coronavirus pandemic

BIOENGINEERING & TRANSLATIONAL MEDICINE (2023)

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Article Medical Laboratory Technology

Comparison of three molecular diagnostic assays for SARS-CoV-2 detection: Evaluation of analytical sensitivity and clinical performance

Ha Nui Kim et al.

Summary: This study compared the performance of three different rRT-PCR assays for SARS-CoV-2 detection. The results showed that the assays had comparable analytical sensitivity and clinical performance. The sensitivity of each target gene varied more than the overall clinical performance.

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2022)

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Article Infectious Diseases

Twelve lateral flow immunoassays (LFAs) to detect SARS-CoV-2 antibodies

Sophie Owen et al.

Summary: The evaluation of 12 LFAs showed varying sensitivity and specificity, with improved sensitivity for IgM/IgG over time since symptom onset. Compared to the EuroImmun IgG ELISA, LFAs exhibited different levels of sensitivity and specificity.

JOURNAL OF INFECTION (2022)

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Article Medicine, General & Internal

Accuracy and ease-of-use of seven point-of-care SARS-CoV-2 antigen-detecting tests: A multi-centre clinical evaluation

Lisa J. Krueger et al.

Summary: This study evaluated the clinical performance and ease-of-use of seven SARS-CoV-2 antigen-detecting rapid diagnostic tests (Ag-RDTs). The results showed high sensitivity and specificity of the Ag-RDTs, especially in early-stage disease and individuals with high viral loads. The tests from Mologic, Bionote, and Standard Q demonstrated good usability.

EBIOMEDICINE (2022)

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Article Microbiology

Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants

Vanessa De Pace et al.

Summary: The diagnostic performance of five qualitative RT-PCR tests was evaluated in comparison with next-generation sequencing (NGS) for the detection of SARS-CoV-2 variants. These tests showed high accuracy and user-friendliness, making them suitable as second-level tests in the routine workflow of SARS-CoV-2 laboratory diagnostics.

MICROORGANISMS (2022)

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Article Biophysics

Rapid detection and tracking of Omicron variant of SARS-CoV-2 using CRISPR-Cas12a-based assay

Yuanhao Liang et al.

Summary: This study designed CRISPR RNAs targeting the spike protein of the Omicron variant and developed a rapid assay based on CRISPR-Cas12a to detect and differentiate Omicron variant from other SARS-CoV-2 variants and common respiratory pathogens. The assay showed a low limit of detection and could be easily implemented in laboratories to monitor and track the spread of the Omicron variant.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Biophysics

SARS-CoV-2 detection using quantum dot fluorescence immunochromatography combined with isothermal amplification and CRISPR/Cas13a

Qin Zhang et al.

Summary: The development of reliable and fast diagnostic devices for COVID-19 is crucial. In this study, a new analysis principle combining reverse transcription and isothermal amplification with CRISPR/Cas13a reaction was proposed. The method showed good sensitivity and specificity in detecting the SARS-CoV-2 gene fragment, and results could be obtained within 1 hour using a portable device. This approach has the potential for point-of-care screening in remote areas with limited resources.

BIOSENSORS & BIOELECTRONICS (2022)

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Article Infectious Diseases

Performance of colorimetric reverse transcription loop-me diate d isothermal amplification as a diagnostic tool for SARS-CoV-2 infection during the fourth wave of COVID-19 in Thailand

Thanyarat Promlek et al.

Summary: The clinical performance of the FastProof 30 min-TTR SARS-CoV-2 RT-LAMP method was validated in this study. The results showed that RT-LAMP had high sensitivity and specificity in detecting COVID-19 viral RNA and has the potential for mass screening of patients with high viral load.

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES (2022)

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Article Immunology

Development and Validation of a Nomogram for Assessing Survival in Patients With COVID-19 Pneumonia

Yi-Min Dong et al.

Summary: Through a study of 628 confirmed cases of COVID-19, it was found that hypertension, neutrophil-to-lymphocyte ratio, and NT-proBNP values were significantly associated with the in-hospital prognosis of patients. The predictive model and nomogram constructed showed good performance in both training and validation cohorts, indicating potential clinical utility in the management of COVID-19.

CLINICAL INFECTIOUS DISEASES (2021)

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Article Infectious Diseases

A highly specific and sensitive serological assay detects SARS-CoV-2 antibody levels in COVID-19 patients that correlate with neutralization

David Peterhoff et al.

Summary: The study developed a specific and sensitive enzyme linked immunosorbent assay (ELISA) for detection of anti-SARS-CoV-2 antibody levels. The ELISA showed high specificity and strong correlation in detecting SARS-CoV-2 antibodies, and was able to generate robust antibody levels 10 days after symptom onset.

INFECTION (2021)

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Article Medical Laboratory Technology

Development and clinical application of a rapid SARS-CoV-2 antibody test strip: A multi-center assessment across China

Mengting Liao et al.

Summary: The SARS-CoV-2 IgG/IgM combined antibody test strip developed in this study showed high sensitivity and specificity in early diagnosis of COVID-19. The positive rate of the kit was 77.3% during the first week after disease onset, reaching 100% since day 9. The results from the kit were consistent with the standard diagnosis, with an AUC of 0.958 and a kappa coefficient of 0.926.

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2021)

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Review Biotechnology & Applied Microbiology

Detection technologies and recent developments in the diagnosis of COVID-19 infection

Praveen Rai et al.

Summary: COVID-19 is a disease caused by SARS-CoV-2 that has spread globally, requiring collaborative efforts for accurate diagnosis and prevention. While RT-qPCR detection is considered the gold standard for early virus detection, challenges such as technical complexity and limited applicability have led to the development of portable POC tests.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2021)

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Article Respiratory System

Development of a SARS-CoV-2 rapid antibody detection kit and study on dynamic changes in antibodies in infected patients

Huihui Wang et al.

Summary: A rapid detection kit for new coronavirus antibodies was developed and tested on SARS-CoV-2-infected and non-infected individuals. The results showed that the positive rates of SARS-CoV-2 IgM and IgG antibodies increased gradually after 1-3 days, 4-6 days, and 7-9 days of admission.

CLINICAL RESPIRATORY JOURNAL (2021)

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Article Microbiology

Saliva Is a Promising Alternative Specimen for the Detection of SARS-CoV-2 in Children and Adults

Rebecca Yee et al.

Summary: Overall concordances for saliva and NPS were 91.0% and 94.7% respectively, with positive percent agreement (PPA) values of 81.4% and 89.7%. Saliva detected 10 additional positive cases compared to NPS. Both saliva and NPS showed comparable performance for symptomatic and asymptomatic pediatric patients not previously diagnosed with COVID-19.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Microbiology

A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein To Facilitate the Detection of SARS-CoV-2

Jose Carlos Ponce-Rojas et al.

Summary: PEARL is an alternative method called precipitation-enhanced analyte retrieval that can quickly and cost-effectively replace commercial RNA extraction kits, offering performance comparable to commercial kits.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Virology

SARS-CoV-2 whole-genome sequencing using reverse complement PCR: For easy, fast and accurate outbreak and variant analysis.

Jordy P. M. Coolen et al.

Summary: This study introduces a novel whole genome sequencing technique for monitoring the transmission and variants of SARS-CoV-2, which proved to be effective in investigating outbreak clusters and identifying new connections amidst the emergence of variants of concern.

JOURNAL OF CLINICAL VIROLOGY (2021)

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Article Biophysics

Development of a SARS-CoV-2-specific biosensor for antigen detection using scFv-Fc fusion proteins

Hye-Yeon Kim et al.

Summary: This study presents the development of a specific LFIA-based biosensor for COVID-19 using phage display technology to generate antibodies targeting SARS-CoV-2 NP antigen, allowing rapid and specific detection of the virus. The newly developed rapid diagnostic test shows potential for use in the diagnosis of COVID-19.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Biophysics

Diagnosis of COVID-19 for controlling the pandemic: A review of the state-of-the-art

Nastaran Taleghani et al.

Summary: Health organizations and countries globally are struggling to control the spread of COVID-19, with scientists working on rapid testing and vaccine development. Fast and accurate diagnosis is crucial for disease prevention and control, and ongoing research and technology advancements are key in this effort.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biophysics

5G-enabled ultra-sensitive fluorescence sensor for proactive prognosis of COVID-19

Jiuchuan Guo et al.

Summary: A 5G-enabled fluorescence sensor was proposed for quantitative detection of SARS-CoV-2 proteins, showing good sensitivity and practicality.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Biochemistry & Molecular Biology

Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera

Piyada Supasa et al.

Summary: Research on the UK-dominant variant B.1.1.7 shows that it is harder to neutralize than the parental virus, but widespread escape from antibodies or monoclonal antibodies has not been observed yet.

CELL (2021)

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Review Pathology

Diagnosis for COVID-19: current status and future prospects

M. D. Alamgir Kabir et al.

Summary: This review discusses the origin of the COVID-19 pandemic, detection methods, and market analysis, emphasizing the importance of developing more effective diagnostic devices and urging testing laboratories to invest in multiplexed and scalable detection tools.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2021)

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Review Microbiology

Performance of Saliva, Oropharyngeal Swabs, and Nasal Swabs for SARS-CoV-2 Molecular Detection: a Systematic Review and Meta-analysis

Rose A. Lee et al.

Summary: Nasopharyngeal (NP) swabs are considered the best sample for diagnosing SARS-CoV-2, but saliva, oropharyngeal (OP), nasal (NS) swabs, and combinations of OP/NS are promising alternatives. While some alternative samples had lower detection rates, a combination of OP/NS showed comparable performance to NP swabs. The absence of RNA extraction (saliva) and use of a more sensitive nucleic acid amplification test (NS) significantly reduced the positive yield of alternative samples.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Virology

Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2

Asmaa M. Altamimi et al.

Summary: The study focuses on the clinical and analytical performance of SARS-CoV-2 (RT-PCR) commercial kits, with four kits showing a sensitivity of 100% and all tested kits scoring above 95% in specificity. Most of the commercially available RT-PCR kits in the study can be used for routine diagnosis of patients with SARS-CoV-2.

JOURNAL OF MEDICAL VIROLOGY (2021)

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Article Immunology

Mutations in SARS-CoV-2; Consequences in structure, function, and pathogenicity of the virus

Behnaz Bakhshandeh et al.

Summary: The third pandemic of coronavirus infection, COVID-19, started recently in China. The main pathogenesis includes severe pneumonia, with various factors potentially influencing mortality and infection rates. Mutations and genetic variabilities of the virus, along with host genetic susceptibility, can impact the clinical features of COVID-19.

MICROBIAL PATHOGENESIS (2021)

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Article Multidisciplinary Sciences

Antibody resistance of SARS-CoV-2 variants B.1.351 and B.1.1.7

Pengfei Wang et al.

Summary: The COVID-19 pandemic has had global repercussions, with promising vaccines and monoclonal antibody therapies. However, newly detected variants of SARS-CoV-2 present challenges to these treatment options.

NATURE (2021)

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Article Biochemistry & Molecular Biology

SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma

Constantinos Kurt Wibmer et al.

Summary: The SARS-CoV-2 virus in the B.1.351 variant discovered in South Africa can evade neutralization by most antibodies when expressed, but does not affect binding by convalescent plasma. This suggests the potential for reinfection with antigenically distinct variants and predicts reduced efficacy of spike-based vaccines.

NATURE MEDICINE (2021)

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Article Multidisciplinary Sciences

Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) assay for on-site diagnosis of SARS CoV-2

Woong Sik Jang et al.

Summary: In this study, three RT-LAMP primer sets were designed for detecting different genes of SARS CoV-2. It was found that the detection limits of two primer sets were low, showing comparable sensitivity to other widely used detection methods.

PLOS ONE (2021)

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Article Chemistry, Analytical

Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus

Chongwen Wang et al.

Summary: The study developed a two-channel fluorescent immunochromatographic assay for early diagnosis of SARS-CoV-2 and FluA, achieving simultaneous detection within 15 minutes with high sensitivity and stability.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

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Review Virology

CRISPR systems: Novel approaches for detection and combating COVID-19

Fatemeh Safari et al.

Summary: The RNA-guided CRISPR enzymes Cas12 and Cas13 are efficient tools for the detection and treatment of RNA viruses, potentially providing a new approach for accurate diagnosis and therapy of diseases like COVID-19 caused by viruses such as SARS-CoV-2.

VIRUS RESEARCH (2021)

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Article Multidisciplinary Sciences

Comparative evaluation of 19 reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

Yajuan Dong et al.

Summary: This study compared the performance of 19 sets of primers in COVID-19 detection and identified 6 sets of primers with faster amplification. Among them, the Set-4 primer set had the highest positive detection rate of SARS-CoV-2.

SCIENTIFIC REPORTS (2021)

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Review Biophysics

CRISPR-based detection of SARS-CoV-2: A review from sample to result

Reza Nouri et al.

Summary: This work reviews and summarizes the applications of CRISPR technology in the context of the COVID-19 pandemic, highlighting its potential for rapid and accurate detection of SARS-CoV-2, as well as providing future prospects for point-of-care molecular diagnosis applications.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Microbiology

Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection

Seyed Hamid Safiabadi Tali et al.

Summary: This paper provides a comprehensive review of technologies and methods used for detecting SARS-CoV-2 during the COVID-19 pandemic, including molecular, antigen-based, and immunological point-of-care testing, diagnostic imaging techniques, and biomarkers. The importance of timing and type of specimen collection is emphasized, along with suggestions for future pandemic preparedness.

CLINICAL MICROBIOLOGY REVIEWS (2021)

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Article Virology

Impaired performance of SARS-CoV-2 antigen-detecting rapid diagnostic tests at elevated and low temperatures

Verena Haage et al.

Summary: This study assessed the analytical sensitivity and specificity of eleven commercially-available SARS-CoV-2 Ag-RDTs under different storage and operational temperatures, finding that elevated temperatures impair sensitivity while low temperatures impair specificity. Recommended storage and operation conditions are essential for successful usage of SARS-CoV-2 Ag-RDTs during the pandemic.

JOURNAL OF CLINICAL VIROLOGY (2021)

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Article Microbiology

A Novel Strategy for the Detection of SARS-CoV-2 Variants Based on Multiplex PCR-Mass Spectrometry Minisequencing Technology

Fei Zhao et al.

Summary: A novel detection strategy using mPCR-MS minisequencing was developed for identifying SARS-CoV-2 variants, demonstrating high specificity and accuracy. This method allows for simultaneous detection of multiple mutation types and can be easily expanded to track new variants. The study highlights the importance of this method for monitoring and managing SARS-CoV-2 infections and emerging variants.

MICROBIOLOGY SPECTRUM (2021)

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Article Microbiology

CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern

Yuanhao Liang et al.

Summary: This study developed a CRISPR-Cas12-based system for the direct detection of SARS-CoV-2 variants, achieved high sensitivity, and successfully distinguished specific mutations in different variants. The approach has the potential for rapid development and implementation in resource-limited settings.

MICROBIOLOGY SPECTRUM (2021)

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Article Biotechnology & Applied Microbiology

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection

Susanna K. Elledge et al.

Summary: A rapid and low-cost solution-based assay has been developed to detect SARS-CoV-2 antibodies in serum, plasma, whole blood, and to some extent saliva, using split luciferase antibody biosensors. This new method generates quantitative results in 30 minutes, simplifying the complexity and improving the scalability of COVID-19 antibody tests. It is suitable for point-of-care testing, population screening, and use in low-resource settings for monitoring host humoral responses to vaccination or viral infection.

NATURE BIOTECHNOLOGY (2021)

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Article Multidisciplinary Sciences

Improved sensitivity, safety, and rapidity of COVID-19 tests by replacing viral storage solution with lysis buffer

Oran Erster et al.

Summary: Using NSLB samples for RT-qPCR tests in COVID-19 patients was found to be significantly more sensitive than using VTM samples, reaching detection threshold 2.1 +/- 0.6 PCR cycles earlier. The NSLB samples, not diluted in lysis buffer before RNA extraction, showed improved sensitivity. Re-extraction of RNA from NSLB samples after 72 hours at room temperature did not affect the sensitivity of detection.

PLOS ONE (2021)

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Article Chemistry, Multidisciplinary

Surveilling and Tracking COVID-19 Patients Using a Portable Quantum Dot Smartphone Device

Yuwei Zhang et al.

Summary: The study developed a portable smartphone-based quantum barcode serological assay device with high clinical sensitivity, enabling real-time surveillance of patients infected with SARS-CoV-2. Compared to lateral flow assays, this device showed significantly higher diagnostic performance and provided instantaneous results for informing patients, physicians, and public health agencies.

NANO LETTERS (2021)

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Article Virology

Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 Detection

Ana Banko et al.

Summary: The study compared the detection performance of three different commercially available SARS-CoV-2 nucleic acid detection kits and found that their results were statistically similar, with high similarity in analytical sensitivities. However, the SanSure Biotech kit showed slightly better diagnostic performance.

VIRUSES-BASEL (2021)

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Article Immunology

RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact

Natali Vega-Magana et al.

Summary: This study developed a fast real-time RT-qPCR assay to detect SARS-CoV-2 mutations with clinical relevance in the Mexican population. The screening of samples from 1060 COVID-19 patients identified the E484K mutation and P.2 Brazilian variant for the first time in Mexico.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

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Article Multidisciplinary Sciences

Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants

Helena de Puig et al.

Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.

SCIENCE ADVANCES (2021)

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Review Medicine, General & Internal

Value of anal swabs for SARS-COV-2 detection: a literature review

Yuliang Wang et al.

Summary: Nucleic acid tests are crucial in diagnosing COVID-19, and universal screening among close contacts and asymptomatic carriers is ongoing. Recent studies have shown that anal swabs in key population screenings can reduce missed diagnoses and aid in tracing infectious sources. The goal is to minimize transmission and protect individuals from COVID-19.

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES (2021)

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Article Immunology

SARS-CoV-2 membrane glycoprotein M antagonizes the MAVS-mediated innate antiviral response

Yu-Zhi Fu et al.

Summary: A novel SARS-related coronavirus (SARS-CoV-2) has emerged as a serious pathogen causing high morbidity and mortality. The membrane glycoprotein M of SARS-CoV-2 was identified as a negative regulator of the innate immune response by impairing MAVS aggregation and downstream signaling, thus evading the innate antiviral response.

CELLULAR & MOLECULAR IMMUNOLOGY (2021)

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Editorial Material Microbiology

Genome Composition and Divergence of the Novel Coronavirus (2019-nCoV) Originating in China

Aiping Wu et al.

CELL HOST & MICROBE (2020)

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Review Cell Biology

CRISPR System: A High-throughput Toolbox for Research and Treatment of Parkinson's Disease

Fatemeh Safari et al.

CELLULAR AND MOLECULAR NEUROBIOLOGY (2020)

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Article Infectious Diseases

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman et al.

EUROSURVEILLANCE (2020)

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Editorial Material Medicine, General & Internal

Characteristics of and Important Lessons From the Coronavirus Disease 2019 (COVID-19) Outbreak in China Summary of a Report of 72 314 Cases From the Chinese Center for Disease Control and Prevention

Zunyou Wu et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2020)

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Article Medicine, General & Internal

Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China

Dawei Wang et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2020)

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Article Virology

Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis

Zhengtu Li et al.

JOURNAL OF MEDICAL VIROLOGY (2020)

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Letter Public, Environmental & Occupational Health

The reproductive number of COVID-19 is higher compared to SARS coronavirus

Ying Liu et al.

JOURNAL OF TRAVEL MEDICINE (2020)

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Editorial Material Medicine, General & Internal

A novel coronavirus outbreak of global health concern

Chen Wang et al.

LANCET (2020)

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Article Multidisciplinary Sciences

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Peng Zhou et al.

NATURE (2020)

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Article Multidisciplinary Sciences

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

Daniel Wrapp et al.

SCIENCE (2020)

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Article Chemistry, Multidisciplinary

Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe Acute Respiratory Syndrome Coronavirus 2 Detection

Guangyu Qiu et al.

ACS NANO (2020)

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Review Chemistry, Multidisciplinary

Diagnosing COVID-19: The Disease and Tools for Detection

Buddhisha Udugama et al.

ACS NANO (2020)

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Article Chemistry, Multidisciplinary

Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor

Giwan Seo et al.

ACS NANO (2020)

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Article Medicine, General & Internal

The Incubation Period of Coronavirus Disease 2019 (COVID-19) From Publicly Reported Confirmed Cases: Estimation and Application

Stephen A. Lauer et al.

ANNALS OF INTERNAL MEDICINE (2020)

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Article Biochemistry & Molecular Biology

Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2

Vanessa Monteil et al.

CELL (2020)

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Article Biochemistry & Molecular Biology

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

Alexandra C. Walls et al.

CELL (2020)

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Article Immunology

Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine

Wanbo Tai et al.

CELLULAR & MOLECULAR IMMUNOLOGY (2020)

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Letter Medical Laboratory Technology

Rapid Detection of COVID-19 Coronavirus Using a Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP) Diagnostic Platform

Lin Yu et al.

CLINICAL CHEMISTRY (2020)

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Article Immunology

Antibody Responses to SARS-CoV-2 in Patients With Novel Coronavirus Disease 2019

Juanjuan Zhao et al.

CLINICAL INFECTIOUS DISEASES (2020)

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Article Immunology

Antibody Detection and Dynamic Characteristics in Patients With Coronavirus Disease 2019

Fei Xiang et al.

CLINICAL INFECTIOUS DISEASES (2020)

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Article Immunology

Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes

Wei Zhang et al.

EMERGING MICROBES & INFECTIONS (2020)

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Review Infectious Diseases

Review of the 2019 novel coronavirus (SARS-CoV-2) based on current evidence

Lisheng Wang et al.

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS (2020)

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Article Computer Science, Information Systems

A Survey of Healthcare Internet of Things (HIoT): A Clinical Perspective

Hadi Habibzadeh et al.

IEEE INTERNET OF THINGS JOURNAL (2020)

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Article Microbiology

Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

Wanbing Liu et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2020)

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Assay Techniques and Test Development for COVID-19 Diagnosis

Linda J. Carter et al.

ACS CENTRAL SCIENCE (2020)

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Quantum Dot Bioconjugates for Diagnostic Applications

Maria Diaz-Gonzalez et al.

TOPICS IN CURRENT CHEMISTRY (2020)

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News Item Biotechnology & Applied Microbiology

Fast, portable tests come online to curb coronavirus pandemic

Cormac Sheridan

NATURE BIOTECHNOLOGY (2020)

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Letter Cell Biology

Single-copy sensitive, field-deployable, and simultaneous dual-gene detection of SARS-CoV-2 RNA via modified RT-RPA

Simin Xia et al.

CELL DISCOVERY (2020)

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Article Biochemistry & Molecular Biology

A serological assay to detect SARS-CoV-2 seroconversion in humans

Fatima Amanat et al.

NATURE MEDICINE (2020)

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Letter Biochemistry & Molecular Biology

The proximal origin of SARS-CoV-2

Kristian G. Andersen et al.

NATURE MEDICINE (2020)

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Article Biochemistry & Molecular Biology

Temporal dynamics in viral shedding and transmissibility of COVID-19

Xi He et al.

NATURE MEDICINE (2020)

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Article Biochemistry & Molecular Biology

Antibody responses to SARS-CoV-2 in patients with COVID-19

Quan-Xin Long et al.

NATURE MEDICINE (2020)

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Article Biotechnology & Applied Microbiology

RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2

Wei E. Huang et al.

MICROBIAL BIOTECHNOLOGY (2020)

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Letter Oncology

How to increase the SARS-CoV-2 detection rate through the nasopharyngeal swab?

Armo De Virgilio et al.

ORAL ONCOLOGY (2020)

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Article Infectious Diseases

Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

C. Yan et al.

CLINICAL MICROBIOLOGY AND INFECTION (2020)

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Article Medical Laboratory Technology

Lab-on-a-Chip Zika Detection With Reverse Transcription Loop-Mediated Isothermal Amplification-Based Assay for Point-of-Care Settings

Sandhya Sharma et al.

ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE (2020)

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Comparative Analysis of Primer-Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)

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ACS INFECTIOUS DISEASES (2020)

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Article Microbiology

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets

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NATURE MICROBIOLOGY (2020)

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Article Microbiology

A Recurrent Mutation at Position 26340 of SARS-CoV-2 Is Associated with Failure of the E Gene Quantitative Reverse Transcription-PCR Utilized in a Commercial Dual-Target Diagnostic Assay

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Article Microbiology

Performance Characteristics of a High-Throughput Automated Transcription-Mediated Amplification Test for SARS-CoV-2 Detection

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JOURNAL OF CLINICAL MICROBIOLOGY (2020)

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Article Medicine, General & Internal

Development and Potential Usefulness of the COVID-19 Ag Respi-Strip Diagnostic Assay in a Pandemic Context

Pascal Mertens et al.

FRONTIERS IN MEDICINE (2020)

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Article Biochemistry & Molecular Biology

Mutations Strengthened SARS-CoV-2 Infectivity

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JOURNAL OF MOLECULAR BIOLOGY (2020)

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Review Biophysics

Detection of COVID-19: A review of the current literature and future perspectives

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BIOSENSORS & BIOELECTRONICS (2020)

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Article Biophysics

Real-time diagnosis of reactive oxygen species (ROS) in fresh sputum by electrochemical tracing; correlation between COVID-19 and viral-induced ROS in lung/respiratory epithelium during this pandemic

Zohreh Sadat Miripour et al.

BIOSENSORS & BIOELECTRONICS (2020)

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Article Biotechnology & Applied Microbiology

Evaluation of SARS-CoV-2 serology assays reveals a range of test performance

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NATURE BIOTECHNOLOGY (2020)

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Letter Medicine, General & Internal

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

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NEW ENGLAND JOURNAL OF MEDICINE (2020)

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Article Chemistry, Multidisciplinary

Emerging Telemedicine Tools for Remote COVID-19 Diagnosis, Monitoring, and Management

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ACS NANO (2020)

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Article Immunology

Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibody Responses in Coronavirus Disease Patients

Nisreen M. A. Okba et al.

EMERGING INFECTIOUS DISEASES (2020)

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Letter Infectious Diseases

Stability of SARS-CoV-2 in different environmental conditions

Alex W. H. Chin et al.

LANCET MICROBE (2020)

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Article Immunology

Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2

Yun Hee Baek et al.

EMERGING MICROBES & INFECTIONS (2020)

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Letter Biotechnology & Applied Microbiology

Establishing CRISPR/Cas13a immune system conferring RNA virus resistance in both dicot and monocot plants

Tong Zhang et al.

PLANT BIOTECHNOLOGY JOURNAL (2019)

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Review Virology

Coronavirus envelope protein: current knowledge

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VIROLOGY JOURNAL (2019)

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Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Patrick Hardinge et al.

SCIENTIFIC REPORTS (2019)

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Review Microbiology

CRISPR-Cas Biology and Its Application to Infectious Diseases

Jeffrey R. Strich et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2019)

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Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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SCIENCE (2018)

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Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

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Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

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Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer

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NATURE (2016)

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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

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SCIENCE (2016)

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Review Biochemistry & Molecular Biology

Epidemiology, Genetic Recombination, and Pathogenesis of Coronaviruses

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TRENDS IN MICROBIOLOGY (2016)

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Review Virology

Structure, Function, and Evolution of Coronavirus Spike Proteins

Fang Li

ANNUAL REVIEW OF VIROLOGY, VOL 3 (2016)

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Review Microbiology

An updated evolutionary classification of CRISPR-Cas systems

Kira S. Makarova et al.

NATURE REVIEWS MICROBIOLOGY (2015)

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Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat

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PLOS ONE (2014)

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Review Chemistry, Multidisciplinary

Functionalizing Nanoparticles with Biological Molecules: Developing Chemistries that Facilitate Nanotechnology

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CHEMICAL REVIEWS (2013)

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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

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CLINICAL CHEMISTRY (2009)

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Quantification of mRNA using real-time RT-PCR

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NATURE PROTOCOLS (2006)

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NUCLEIC ACIDS RESEARCH (2000)

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