4.5 Article

PDGFA-associated protein 1 is a novel target of c-Myc and contributes to colorectal cancer initiation and progression

期刊

CANCER COMMUNICATIONS
卷 42, 期 8, 页码 750-767

出版社

WILEY
DOI: 10.1002/cac2.12322

关键词

carcinogenesis; colorectal cancer; FRA-1; PDAP1; c-Myc

类别

资金

  1. National Key Research and Development Program of China [2018YFA0109000, 2019YFC1316303]
  2. National Postdoctoral Program for Innovative Talents [BX20180376]
  3. Natural Science Foundation of Shaanxi Province [2020SF-252]
  4. Basic Research Plan of Natural Science in Shaanxi Province [2020JM-326]
  5. National Natural Science Foundation of China [31601127, 82173244]

向作者/读者索取更多资源

This study identified PDAP1 as a key player in colorectal cancer (CRC) development. PDAP1 overexpression was associated with cell proliferation, migration, invasion, and metastasis in CRC. Inhibiting PDAP1 may be an effective strategy for treating CRC patients with PDAP1 overexpression.
Background The mechanism underlying colorectal cancer (CRC) initiation and progression remains elusive, and overall survival is far from satisfactory. Previous studies have shown that PDGFA-associated protein 1 (PDAP1) is upregulated in several cancers including CRC. Here, we aimed to identify the cause and consequence of PDAP1 dysregulation in CRC and evaluate its role as a potential therapeutic target. Methods Multi-omics data analysis was performed to identify potential key players in CRC initiation and progression. Immunohistochemistry (IHC) staining was applied to determine the expression pattern of PDAP1 in CRC tissues. Pdap1 conditional knockout mice were used to establish colitis and CRC mouse models. RNA sequencing, a phosphoprotein antibody array, western blotting, histological analysis, 5-bromo-2 '-deoxyuridine (BrdU) incorporation assay, and interactome analysis were applied to identify the underlying mechanisms of PDAP1. A human patient-derived xenograft (PDX) model was used to assess the potential of PDAP1 as a therapeutic target. Results PDAP1 was identified as a potential key player in CRC development using multi-omics data analysis. PDAP1 was overexpressed in CRC cells and correlated with reduced overall survival. Further investigation showed that PDAP1 was critical for the regulation of cell proliferation, migration, invasion, and metastasis. Significantly, depletion of Pdap1 in intestinal epithelial cells impaired mucosal restitution in dextran sulfate sodium salt-induced colitis and inhibited tumor initiation and growth in colitis-associated cancers. Mechanistic studies showed that c-Myc directly transactivated PDAP1, which contributed to the high PDAP1 expression in CRC cells. PDAP1 interacted with the juxtamembrane domain of epidermal growth factor receptor (EGFR) and facilitated EGFR-mitogen-activated protein kinase (MAPK) signaling activation, which resulted in FOS-related antigen 1 (FRA-1) expression, thereby facilitating CRC progression. Notably, silencing of PDAP1 could hinder the growth of patient-derived xenografts that sustain high PDAP1 levels. Conclusions PDAP1 facilitates mucosal restitution and carcinogenesis in colitis-associated cancer. c-Myc-driven upregulation of PDAP1 promotes proliferation, migration, invasion, and metastasis of CRC cells via the EGFR-MAPK-FRA-1 signaling axis. These findings indicated that PDAP1 inhibition is warranted for CRC patients with PDAP1 overexpression.

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