期刊
JOURNAL OF EUKARYOTIC MICROBIOLOGY
卷 63, 期 6, 页码 760-770出版社
WILEY
DOI: 10.1111/jeu.12323
关键词
cox1 gene; cryptic species; SSU rRNA gene; Tetrahymena pyriformis complex
类别
资金
- Natural Science Foundation of China [31401963, 31522051]
- Research on Key Techniques of Water Pollution Disposal and Comprehensive Environmental Control under the Sever Anthropogenic Disturbance
- Fundamental Research Funds for the Central Universities [201564022]
- Scientific Program of Tianjin City [15YFYBJC00710]
A cryptic species of the Tetrahymena pyriformis complex, Tetrahymena australis, has been known for a long time but never properly diagnosed based on taxonomic methods. The species name is thus invalid according to the International Code of Zoological Nomenclature. Recently, a population isolated from a freshwater lake in Wuhan, China was investigated using live observations, silver staining methods and gene sequence data. This organism can be separated from other described species of the T. pyriformis complex by its relatively small body size, the number of somatic kineties and differences in sequences of two genes, namely the small subunit ribosomal RNA (SSU rRNA) and the mitochondrial cytochrome c oxidase subunit I (cox1). We compared the SSU rRNA gene sequences of all available Tetrahymena species to reveal the nucleotide differences within this genus. The sequence of the Wuhan population is identical to two sequences of a previously isolated strain of T. australis (ATCC # 30831). Phylogenetic analyses indicate that these three sequences (X56167, M98015, KT334373) cluster with Tetrahymena shanghaiensis (EF070256) in a polytomy. However, sequence divergence of the cox1 gene between the Wuhan population and another strain of T. australis (ATCC # 30271) is 1.4%, suggesting that these may represent different subspecies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据