Co-assembled Coiled-Coil Peptide Nanotubes with Enhanced Stability and Metal-Dependent Cargo Loading

Peptide nanotube biomaterials are attractive for their range of applications. Herein, we disclose the co-assembly of coiled-coil peptides, one with ligands for metal ions that demonstrate hierarchical assembly into nanotubes, with spatial control of the metal-binding ligands. Enhanced stability of the nanotubes to phosphate-buffered saline was successfully accomplished in a metal-dependent fashion, depending on the levels and placement of the ligand-containing coiled-coil peptide. This spatial control also allowed for site-specific labeling of the nanotubes with His-tagged fluorophores through the length of the tubes or at the termini, in a metal-dependent manner.

Automated summary

This study revealed the co-assembly of coil-coil peptides with metal ion ligands for hierarchical assembly into nanotubes with spatial control, enhancing stability in phosphate-buffered saline in a metal-dependent manner. Spatial control also allowed for site-specific labeling with His-tagged fluorophores through a metal-dependent manner.