Sodium Chloride (NaCl)-Induced Physiological Alteration and Oxidative Stress Generation in Pisum sativum (L.): A Toxicity Assessment

Salinity stress has a deleterious impact on plant development, morphology, physiology, and biochemical characteristics. Considering the NaClinduced phytotoxicity, current investigation was done to better understand the salttolerant mechanisms using Pisum sativum L. (pea) as a model crop. Generally, NaCl resulted in a progressive decrease in germinative attributes and physiological and biochemical parameters of P. sativum (L.). The 400 mM NaCl level had a higher detrimental effect and reduced the germination rate, plumule, radicle length, and seedling vigor index (SVI) by 78, 89, 84, and 77%, respectively, under in vitro. Furthermore, after 400 mM NaCl exposure, physiological and enzymatic profiles like root dry biomass (71%) chl-a (66%), chl-b (54%), total chlorophyll (45%), and nitrate reductase activity (NRA) (59%) of peas were decreased. In addition, a NaCl doserelated increase in soluble protein (SP) and sugar (SS), Na+ and K+ ions, and stressor metabolites was recorded. For instance, at 400 mM NaCl, SP, SS, Na+ ion, K+ ion, root proline, and malondialdehyde (MDA) contents were significantly and maximally elevated by 65, 33, 84, 79, 85, and 89%, respectively, compared to the control (0 mM NaCl). Data analysis indicated that greater doses of pesticides dramatically increased reactive oxygen species (ROS) levels and induced membrane damage through production of thiobarbituric acid reactive substances (TBARS), as well as increased cell injury. To deal with NaCl-induced oxidative stress, plants subjected to higher salinity stress showed a considerable build-up in antioxidant levels. As an example, ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were maximally and significantly (p <= 0.05) increased by 68, 80, 74, and 58%, respectively, after 400 mM NaCl exposure. The propidium iodide (PI)-stained and NaCl-treated plant roots corroborated the damaging effect of salinity-induced stress on the cell membrane, which was observed under a confocal laser microscope (CLSM). The cells exposed to 400 mM NaCl had maximum fluorescence intensity, indicating that higher level of salts can cause pronounced cell damage and reactive oxygen species (ROS) generation. The increases in superoxide ion (O2-) and hydrogen peroxide (H2O2) content in NaCl-treated plant tissues indicated the elevation of ROS with increasing salt levels. This finding revealed that salt stress can cause toxicity in plants by causing alteration in metabolic activity, oxidative injury, and damage to cell membrane integrity.

Automated summary

Salt stress negatively affects germination and physiological parameters of peas. Peas subjected to high salt stress show oxidative stress and respond by increasing antioxidant levels. Salt stress also leads to membrane damage.