期刊
PLANTS-BASEL
卷 11, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/plants11131699
关键词
Lantana camara; Lantana montevidensis; antioxidant; anti-inflammatory; cytotoxicity; LC-MS-MS
资金
- Ministry of Science and Technology [MOST 111-2321-B-182-001, MOST 109-2320-B-650-001-MY3, MOST 108-2320-B-255-003-MY3, MOST 109-2327-B-255-001]
- Chang Gung University of Science and Technology [ZRRPF3L0091]
- Chang Gung Memorial Hospital [CMRPF1J0051similar to3, CORPF1L0011, CMRPF1L0071, BMRP450]
The methanolic extracts of Lantana camara and Lantana montevidensis leaves show potential antioxidant, anti-inflammatory, and anticancer activities, inhibiting free radicals, cancer cell proliferation, reducing metastasis, and inducing apoptosis.
Lantana camara L. and Lantana montevidensis Briq. (F. Verbenaceae) are invasive ornamental weeds native to the tropical regions of Africa and America. The leaves of both species have been traditionally used as infusions for treating fever, rheumatism, and cancer. LC-MS-MS-guided profiling of the methanolic extracts of the leaves of L. camara and L. montevidensis growing in Egypt led to the putative identification of 59 compounds belonging to terpenoids, flavonoids, iridoid glycosides, phenolic acids, and their derivatives. The in vitro antioxidants and anti-inflammatory and anticancer activities of the two extracts were investigated. L. camara and L. montevidensis inhibited DPPH center dot (IC50 = 34.01 +/- 1.32 and 47.43 +/- 1.74 mu g/ mL), ABTS (IC50 = 30.73 +/- 1.42 and 40.37 +/- 1.51 mu g/mL), and superoxide anion (IC50 = 1.57 +/- 0.19 and 1.31 +/- 0.14 mu g/mL) free radicals. A potent antiinflammatory effect was observed for both species through the inhibition of elastase release in fMLF/CB-induced human neutrophils (IC50 = 2.40 +/- 0.16 and 1.90 +/- 0.07 mu g/mL). The extracts showed significant cytotoxic activity against a panel of cancer cell lines with the most potent activity against Caco cells (IC50 = 45.65 +/- 1.64 and 40.67 +/- 1.52 mu g /mL for L. camara and L. montevidensis, respectively). Western blotting supported by FACS analysis revealed that the extracts inhibited cancer cell proliferation, reduced metastasis, and induced apoptosis resulting in cell cycle arrest. This was achieved via increasing mRNA and protein expressions of p53 and GSK-313 as well as decreasing the expression of PI3K, Akt, and cyclin D1.
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